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Test method for trazodone
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A detection method, the technology of trazodone, which is applied in the detection field of trazodone, can solve the problems of time-consuming and long sample detection time, etc.
Active Publication Date: 2022-07-12
合肥和合医疗科技有限公司
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However, the detection by this method usually requires complex pretreatment of the sample to be tested, including complex operations such as drying with nitrogen and adding complex solutions, which consumes a lot of time, resulting in a longer sample detection time
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Embodiment 1
[0103] Example 1: Preparation of standard solutions of series concentrations
[0105] Accurately weigh 5 mg of standard trazodone into a 5 mL volumetric flask, dissolve in methanol, and dilute to 5 mL to obtain a standard stock solution, which is stored at -80°C.
[0106] (b) Preparation of standard working solution
[0107] Take an appropriate amount of the standard stock solution in step (a), dilute and mix with 70% methanolaqueous solution as a diluent to obtain a series of standard mixed intermediate solutions containing 500-30000ng / mL trazodone, and store at -80 °C ;
[0108] Among them, the standard working solutions of different concentrations are a series of solutions containing trazodone: 500ng / mL, 2000ng / mL, 5000ng / mL, 10000ng / mL, 15000ng / mL, 20000ng / mL, and 30000ng / mL.
[0109] (c) Preparation of internal standard stock solution
[0110] Take 5 mg of 2-naphthol standard substance and put it in a 5 mL volumetr...
[0116] The seven standard solutions in Example 1 were respectively detected by a liquid chromatograph, and the chromatograms of the standard solutions of seven different concentrations of trazodone were obtained.
[0117] From the chromatograms of the standard solutions of trazodone above, the corresponding peak areas of trazodone and the internal standard in the seven standard solutions were obtained, respectively, and the peaks of trazodone obtained from the chromatograms of the standard solutions of each concentration above were obtained. The ratio of the area to the chromatographic peak area of the internal standard is taken as the ordinate y1 of the standard curve equation, and the concentration in the above-mentioned trazodone standard working solution and the concentration of the internal standard are taken as the abscissa x1 of the standard curve equation. The data of different concentrations are subjected to li...
Embodiment 3
[0126] Example 3: Treatment of samples to be tested
[0127] 3.1 Take at least 5 mL of blood to be processed, centrifuge at 3500 rpm for 10 min, take the supernatant serum or plasma as the first supernatant, and store the above-mentioned serum or plasma at -20°C for use before analysis.
[0128] 3.2 Use a pipette to pipette 10 μL of the internal standard working solution in Example 1 into a 1.5 mL centrifuge tube, then add 100 μL of serum or plasma from step 3.1, vortex and mix for 1 min at 2000 rpm, and add proteinprecipitation Add 1000 μL of acetonitrile solution, vortex mixing at 2000 rpm for 5 min, and then centrifuge at 14000 rpm for 10 min, the obtained second supernatant is the sample to be tested.
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Abstract
The present invention provides a method for detecting trazodone, comprising: preparing standard solutions with at least three concentrations of trazodone and an internal standard, wherein the amount of the internal standard in the standard solution is the same; using a liquid chromatograph under detection conditions Detecting each standard solution down to obtain the first detection result corresponding to the standard solution; Fit the standard curve equation of trazodone according to each first detection result, the concentration of trazodone in the standard solution and the concentration of the internal standard substance; Take the first supernatant after centrifugation of the sample to be processed; add the internal standard to the first supernatant, vortex to mix, add protein precipitant in sequence, and perform proteinprecipitation on the first supernatant to obtain the sample to be tested. sample; use a liquid chromatograph to detect the sample to be tested under detection conditions to obtain a second detection result of the sample to be tested; based on the standard curve equation and the second detection result, obtain the concentration of trazodone in the sample to be tested. This solution can shorten the sample detection time.
Description
technical field [0001] The invention relates to the technical field of biological detection, in particular to a detection method of trazodone. Background technique [0002] Trazodone is a triazolepyridine derivative with central sedative and slightly muscle-relaxing effects. [0003] At present, the commonly used method for detecting the content of trazodone in a sample is high performance liquid chromatography. However, the detection by this method usually requires more complicated pretreatment of the sample to be tested, including complicated operations such as drying with nitrogen gas and adding and reconstituting the solution, which takes a lot of time, resulting in a long sample detection time. SUMMARY OF THE INVENTION [0004] The invention provides a detection method for trazodone, which can shorten the sample detection time. [0005] In order to solve the above problems, the embodiment of the present invention provides a detection method of trazodone, including:...
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