A kind of preparation method of double protein hydrogel

A hydrogel and double protein technology, applied in the field of medical biomaterials, can solve the problems of low cell survival rate, inability to precisely control the properties of gel and hydrogel, poor mechanical properties, etc., and achieve high biological activity and high cell encapsulation The effect of rate and cell release ability, good biocompatibility

Active Publication Date: 2021-11-02
广州达康基因技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many currently available injectable hydrogels suffer from poor mechanical properties, low cell viability, or inability to precisely control gel and hydrogel properties

Method used

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  • A kind of preparation method of double protein hydrogel
  • A kind of preparation method of double protein hydrogel
  • A kind of preparation method of double protein hydrogel

Examples

Experimental program
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Effect test

Embodiment 1

[0045] This example provides a method for preparing a double protein hydrogel. The preparation method of the double protein hydrogel of the present embodiment comprises the following steps:

[0046] (1) Preparation of methacrylated gelatin (GelMA)

[0047] Dissolve 10g of gelatin in 100mL of deionized water, add 6mL of methacrylic anhydride, and react at room temperature for 12 hours. After the reaction is completed, put it into a dialysis bag with a molecular weight cut-off of 3500, and dialyze it with distilled water for 5-7 days. Freeze-dry at ℃ to get methacrylated gelatin (GelMA);

[0048] (2) Preparation of methacrylated sericin (SerMA)

[0049] Take 20g of silkworm cocoons and wash them with deionized water to remove impurities, dry them at 25°C, and wash them with 0.1mol / L Na 2 CO 3 The solution (1000mL) was soaked and boiled for 1h, and the boiled solution was taken out every half hour and filtered through a magic filter cloth to remove impurities to obtain a seri...

Embodiment 2

[0053] This example provides a method for preparing a double protein hydrogel. The preparation method of the double protein hydrogel in this embodiment is the same as in Example 1 except that the concentration of GelMA in the reaction raw material solution obtained in step (3) is 5%, and the concentration of SerMA is 6%.

Embodiment 3

[0055] This example provides a method for preparing a double protein hydrogel. The preparation method of the double protein hydrogel in this embodiment is the same as that in Example 1 except that the concentration of GelMA in the reaction raw material solution obtained in step (3) is 5%, and the concentration of SerMA is 9%.

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Abstract

The invention provides a method for preparing a double protein hydrogel, which belongs to the technical field of medical biomaterials. The invention dissolves methacrylated gelatin, methacrylated sericin and a blue light initiator in water, and then irradiates with blue light to obtain a double protein hydrogel. The present invention can adjust the structure, mechanical properties, hydrogel properties and degradability of the obtained double protein hydrogel by adjusting the concentration and ratio of methacrylated gelatin and methacrylated sericin in the reaction solution, and the obtained The double protein hydrogel has good biocompatibility, can support the adhesion of stem cells, has a high cell encapsulation rate and cell release ability when used to load cells, and the released cells can maintain high biological activity. The release of stem cells lasts for more than 7 days, which is suitable for biomedicine, tissue engineering and other fields.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a preparation method of a double protein hydrogel. Background technique [0002] Stem cells have shown great potential in the treatment of a variety of injuries and diseases due to their pluripotency, ability to release growth factors, and ability to regulate inflammation. Local injection, which delivers cells directly to the site of injury with minimal invasiveness, is the usual delivery strategy. Local injections are one of the main reasons for stem cell therapy. However, the low cell engraftment efficiency of the injection method greatly limits the clinical promotion of stem cell therapy. One of the main reasons for the low cell retention is the lack of a 3D matrix to support the survival, migration, and development of transplanted cells. [0003] To address this issue, a variety of injectable hydrogel systems have been developed that can provide me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F299/00C08F2/48C08J3/075C08L55/00
CPCC08F2/48C08F299/00C08J3/075C08J2355/00
Inventor 肖扬邓凯贤王雪峰郭瑞
Owner 广州达康基因技术有限公司
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