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Application of AAT protein expressed by pulmonary alveolar lavage fluid of lung cancer patient

A technology for alveolar lavage fluid and patients, which is applied in the direction of material inspection products, measuring devices, instruments, etc., and can solve problems such as correlations to be evaluated

Pending Publication Date: 2021-02-09
BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the correlation between the level of AAT in the alveolar lavage fluid of patients with lung cancer and the disease remains to be evaluated

Method used

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  • Application of AAT protein expressed by pulmonary alveolar lavage fluid of lung cancer patient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1 Alveolar lavage fluid collection

[0011] Patients were first under local anesthesia with 2% lidocaine, followed by bronchoscopy using a fiberoptic bronchoscope (OlympusExera BF 240). To prevent any possibility of blood contamination, flushing of biopsy or brush specimen collection is required. The diseased side bronchi were rinsed twice by 50 ml of sterile saline, then this solution was slowly placed in glass tubes and placed in ice water for rinsing. Recover 40ml of alveolar lavage fluid. Immediately after separation, the lavage fluid was spun at 1500g for 10 minutes, and then the supernatant was frozen at -80°C.

Embodiment 2

[0012] Example 2 Detection of AAT protein

[0013] The concentration of AAT in alveolar lavage fluid was detected by enzyme-linked immunosorbent assay kit. BALF samples were diluted 500-fold and incubated in ELISA plates. Optical density was measured at 450 nm with a model 680 microplate analyzer (BIO-RAD). Using the concentrations of standard samples and the corresponding optical density values, draw a standard curve for each plate. At the same time, positive and negative controls were used in the test to improve the validity of the method.

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Abstract

The invention provides a method for detecting pulmonary alveolar lavage fluid protein, and successfully detects expression characteristics of alpha-1-antitrypsin (AAT) in pulmonary alveolar lavage fluid of normal people and lung cancer patients. Researches prove expression characteristics of AAT in alveolar lavage fluid of normal people and lung cancer patients, and prove that the alveolar lavagefluid AAT has a diagnostic value on disease conditions of the patients.

Description

technical field [0001] The invention relates to detecting the alveolar lavage fluid protein of normal people and lung cancer patients, and confirms that the content of AAT in the alveolar lavage fluid of lung cancer patients is significantly higher than that of normal people. Background technique [0002] Lung cancer is the leading cause of cancer death worldwide, accounting for more than a quarter of all cancer deaths. Lung cancer kills 400,000 people every year. At present, the main means of lung cancer treatment is still surgical resection, but once the disease develops to the metastatic stage, surgical resection is usually ineffective, and the prognosis of advanced patients is poor. Chemotherapy generally cannot treat patients with metastatic lung cancer, and the 5-year survival rate of patients is less than 15%. At present, there is no clear best way to treat lung cancer. The poor survival rate of lung cancer patients is attributed to the lack of early diagnosis and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6884G01N2800/12
Inventor 张曼王巍伟
Owner BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY