Application of timosaponin N in preparation of medicine for preventing and treating pulmonary fibrosis

A technology of timosaponin and pulmonary fibrosis, applied in the field of medicine, can solve the problems of prevention and treatment of pulmonary fibrosis without timosaponin N, achieve important social and economic benefits, improve the degree of pulmonary fibrosis, and improve body weight

Active Publication Date: 2020-08-11
HEILONGJIANG UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Application of timosaponin N in preparation of medicine for preventing and treating pulmonary fibrosis
  • Application of timosaponin N in preparation of medicine for preventing and treating pulmonary fibrosis
  • Application of timosaponin N in preparation of medicine for preventing and treating pulmonary fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preparation of total extract of Anemarrhena 90% ethanol and preparation and characterization of timosaponin N

[0030] Take 2.0kg of Anemarrhena medicinal material, reflux extraction with 20L 90% (v / v) ethanol for 3 times, reflux for 2 hours each time, filter, and combine the filtrates to obtain the total extract of Anemarrhena 90% ethanol, reclaim ethanol, and concentrate under reduced pressure to 5L, reserve a part of the concentrate for subsequent administration.

[0031] Add 15L of water to dilute the remaining concentrate to a volume concentration of 0.1g crude drug / mL, filter the solution, and the filtrate is ready for loading. Pack the pre-treated macroporous adsorption resin HP20 into a column (5L), and after standing to balance, the filtrate is loaded. After loading the sample, wash with 6 times column volume (6BV) of water to remove impurities, then use 6BV of 30% (v / v) ethanol to remove impurities, use 6BV of 50% (v / v) ethanol to elute, and finall...

Embodiment 2

[0033] Example 2: Pharmacodynamic study of timosaponin N against pulmonary fibrosis

[0034] 1. Purpose of the experiment

[0035]To investigate the effect of timosaponin N on the model of pulmonary fibrosis induced by bleomycin in rats

[0036] 2. Experimental materials

[0037] 2.1 Animals: SPF grade SD rats, male, weighing 200±20g, purchased from Beijing Huafukang Biotechnology Co., Ltd., license number: SCXK (Beijing) 2018-0006.

[0038] 2.2 Main instruments: analytical balance, electronic balance, 2mL syringe, gavage needle, animal cage, water bath, ultraviolet spectrophotometer, microplate reader, etc.

[0039] 2.3 Main drugs and reagents: bleomycin (Nippon Kayaku Co., Ltd.), pirfenidone (Beijing Condini Pharmaceutical Co., Ltd.), hydroxyproline kit (Nanjing Jiancheng Bioengineering Research Institute), TGF- β and TNF-α kit (R&DSystems).

[0040] 3. Experimental method

[0041] 3.1 Establishment and administration of rat pulmonary fibrosis model

[0042] Raise SD r...

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Abstract

The invention relates to an application of timosaponin N in preparation of a medicine for preventing and treating pulmonary fibrosis. In the embodiment of the invention, the timosaponin N can remarkably improve the weight and lung index of a bleomycin pulmonary fibrosis model rat, and remarkably reduce biochemical indicators closely related to the pulmonary fibrosis, such as the content of hydroxyproline in lung tissues and the levels of TGF-beta and TNF-alpha in pulmonary alveolar lavage fluid. In addition, the indexes related to the pulmonary fibrosis can be further improved synergisticallyby combining timosaponin N with pirfenidone. Therefore, the timosaponin N or the combination of the timosaponin N and the pirfenidone can effectively improve a pulmonary fibrosis degree of a pulmonaryfibrosis model rat, have the prospect of being developed into the anti-pulmonary fibrosis medicine, and have important social benefits and economic benefits.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a new application of timosaponin N, in particular to the application of timosaponin N in the preparation of medicines for preventing and treating pulmonary fibrosis. Background technique [0002] Interstitial lung disease (ILD) is a disease spectrum composed of many heterogeneous diseases caused by alveolar wall as the main lesion. Pulmonary fibrosis is the ultimate manifestation of various interstitial lung diseases. Due to alveolar damage, repeated damage and repair of lung tissue, fibroblast proliferation and activation, and a large amount of extracellular matrix deposition, a large number of collagen fibers are eventually deposited in lung tissue. Idiopathic pulmonary fibrosis (IPF) is the most common type of pulmonary fibrosis, accounting for more than half of patients with pulmonary fibrosis, and it is also the fastest developing and deteriorating disease among various fibrosis disease...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P11/00
CPCA61K31/7048A61P11/00
Inventor 陈宏李全
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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