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Fluorescent probe for detecting dodine in water as well as preparation method and application of fluorescent probe

A technology of fluorescent probes and Duogudine, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of complex detection and achieve simple, sensitive, fast and efficient detection process

Active Publication Date: 2021-02-12
GUIZHOU EDUCATION UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current detection of dodine is very complicated, therefore, there is an urgent need to develop simple, rapid and sensitive analytical methods to identify and quantify specific pesticide residues

Method used

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  • Fluorescent probe for detecting dodine in water as well as preparation method and application of fluorescent probe
  • Fluorescent probe for detecting dodine in water as well as preparation method and application of fluorescent probe
  • Fluorescent probe for detecting dodine in water as well as preparation method and application of fluorescent probe

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: a kind of method for preparing the fluorescent probe that detects dodine in water, concrete steps are as follows:

[0035] (1) Dissolve ten-membered melon rings in water to obtain a concentration of 1×10 -4 mol / L solution A;

[0036] (2) Dissolve methylacridine in water to obtain a concentration of 1×10 -3 mol / L into solution B;

[0037] (3) Mix solution A and solution B, control the molar ratio of ten-membered cucurbit ring and methylacridine to 1:2, and react at room temperature to obtain an aqueous solution of a fluorescent probe for detecting dodine in water.

Embodiment 2

[0039] 1. Preparation of standard solution of pesticide Duoguudin:

[0040] Accurately weigh an appropriate amount of Duoguodin, dissolve it in secondary water with pH=7 and set the volume to 10mL to obtain a concentration of 2.00×10 -1 mol / L standard solution of pesticide Duofruit;

[0041] 2. Draw the standard curve:

[0042] Take a quartz fluorescence cuvette, add 1.0×10 -4 After 3000 μL of mol / L fluorescent probe solution (the concentration of fluorescent probe is 2×10 -5 mol / L), accurately add 2.00×10 -1 1.0 μL of mol / L standard solution of pesticide Duoguudin was placed in a cuvette, stirred evenly, and the excitation wavelength was fixed at 364nm for fluorescence emission spectrum measurement;

[0043] According to the above operation, continuously add quantitative 1.0 μL pesticide Duoguudin standard solution to the above 3000 μL probe solution, and measure a series of fluorescence curves at 364 excitation wavelength until the ordinate value of the fluorescence curv...

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Abstract

The invention discloses a fluorescent probe for detecting dodine in water as well as a preparation method and application of the fluorescent probe. The molecular formula of the fluorescent probe is C88H84O20N42Br2, and the fluorescent probe is prepared from a cucurbit[10]uril aqueous solution and a methyl acridine aqueous solution. The application comprises the steps: during detection, firstly, taking the fluorescent probe, and adding secondary water for dilution to obtain a fluorescent probe solution; adding to-be-detected water into the prepared fluorescent probe solution, standing for 10-20minutes, carrying out fluorescence emission spectrum measurement at a fixed excitation wavelength of 364 nm, and drawing a change curve of fluorescence intensity at the excited laser wavelength; andfinally, calculating the change value of the fluorescence emission spectrum intensity under the corresponding 485 nm before and after the to-be-detected water is added into the fluorescence probe solution according to the curve, and when the fluorescence emission spectrum intensity under the corresponding 485 nm before and after the to-be-detected water is added is enhanced, indicating that the to-be-detected water contains dodine. The method has the characteristics that the dodine detection process is simple, and the detection is rapid and sensitive.

Description

technical field [0001] The invention relates to a fluorescent probe and its preparation method and application, in particular to a fluorescent probe for detecting dodine in water and its preparation method and application. Background technique [0002] The use of pesticides in agriculture is necessary to eliminate many pests that can damage crops and to improve the quality of the food produced. On the other hand, the presence of pesticide residues in the environment poses a high risk to human health due to their increasing usage. [0003] Dodine (dodecidine acetate) is a widely used fungicide with low toxicity and protective properties. It is used to control many major mold diseases on fruit trees, vegetables, nuts, ornamentals and shade trees. Its main rationale as a biocide is that it can easily adsorb to the surface of negatively charged microorganisms, penetrate their membranes and disrupt their cellular structure. Therefore, it has a bactericidal effect and eliminate...

Claims

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Application Information

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IPC IPC(8): G01N21/64C07D219/02C07D487/22C09K11/06
CPCG01N21/6402G01N21/643C07D487/22C07D219/02C09K11/06C09K2211/1029C09K2211/1074
Inventor 张桂玲罗阳肖昕罗绪强张威胥卫涛
Owner GUIZHOU EDUCATION UNIV
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