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Method for screening material suppressing fine dust-induced retinal cell damage and composition for suppressing fine dust-induced retinal cell damage

A technology of retinal cells and screening methods, applied in the field of retinal cell damage compositions, to achieve the effect of inhibiting damage

Active Publication Date: 2021-02-12
AMOREPACIFIC CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, when such dust damages retinal pigment epithelial cells, there is a risk of causing retinal pigment epithelial cell-related diseases, such as retinitis pigmentosa, age-related macular degeneration, diabetic retinopathy, etc.

Method used

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  • Method for screening material suppressing fine dust-induced retinal cell damage and composition for suppressing fine dust-induced retinal cell damage
  • Method for screening material suppressing fine dust-induced retinal cell damage and composition for suppressing fine dust-induced retinal cell damage
  • Method for screening material suppressing fine dust-induced retinal cell damage and composition for suppressing fine dust-induced retinal cell damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Preparation and immunofluorescent staining of micropigmented retinal cells

[0065] In a 5% (v / v) CO incubator, at a temperature of 37°C, 10% (v / v) of fetal bovine serum (FBS) and 1% of penicillin-streptomycin (Invitrogen) were added. Retinal pigment epithelial cells (human telomerase-immortalized retinal pigmented epithelial cells, htRPE cells) purchased from ATCC (American Type Culture Collection) were cultured in DMEM (Dulbecco's modified Eagle`s medium) in Carlsbad, California, USA.

[0066] After the human epidermal normal keratinocytes were transferred to Lab-TexTM 2-well chamber slides (Tnermo Scientific Company, Waltham, Massachusetts, U.S.A.) at a cell number of 0.5×105 per well, the cultured retinal pigment epithelial cells were Inoculate (6x104 cell number / well) on a 12-well plate, and after 24 hours, use dust PM10 (ERM-CZ100, sample number: 0504) purchased from ERM (European Reference Materials) at 100 μg / ml and 300 μg / ml respectively concentration, then ri...

experiment Embodiment 1

[0083] [Experimental Example 1] Recovery of retinal pigment epithelial cells caused by fine dust by formation of primary cilia

[0084] The primary cilium is an intracellular organ with a bipolar structure, and the combination (formation of cilia) and disassembly of cilia are interconnected through various causes such as cell cycle, polarity, vertebral development, and genetic diseases. It is known that primary cilia play an important role in the function of retinal pigmented epithelial cells. Specifically, retinal pigment epithelial cells play an important role in absorbing light and transporting substances in the eye. In order to complete this function well, good development of primary cilia is required. Therefore, changes in the expression of primary cilia when the retinal pigment epithelial cells were treated with fine dust were experimentally confirmed.

[0085] RPE cells were cultured in serum-free medium to confirm whether serum starvation induces primary cilia formati...

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Abstract

The present invention relates to a method for screening a material suppressing fine dust-induced retinal cell damage, the method comprising a step of identifying a relative degree of formation of primary cilia in retinal cells. According to an aspect of the present invention, a material suppressing fine dust-induced retinal cell damage can be effectively screened and excavated, thereby allowing aplan to develop a business related to eye health associated with fine dust removal.

Description

technical field [0001] The present invention relates to a method for screening substances for inhibiting retinal cell damage caused by fine dust, and specifically, to a method for screening substances for inhibiting retinal cell damage caused by fine dust by confirming the degree of formation of primary cilia as cellular micro-organs. A method for a substance that damages retinal cells, and a composition for inhibiting damage to retinal cells caused by fine dust. Background technique [0002] Retinal Pigment Epithelium cells (RPE cells) refer to pigment-containing cells that exist outside the sensory nerve area of ​​the retina. They exist in the outermost layer of the retina in a single layer and participate in the development of the retina to protect the vision The role of rods and cones. [0003] Specifically, the functions of retinal pigment epithelial cells include: eliminating photooxidative stress (Photooxidative Stress) by absorbing scattered light, thereby participa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/68A23L33/13A61K31/7105A61K31/713A61K48/00A61P27/02
CPCA23L33/13A61K31/7105A61K31/713A61K48/00A61P27/02G01N33/50G01N33/68G01N33/5044G01N33/5076G01N33/6893A23V2002/00
Inventor 崔贤贞金亨俊李泰龙赵桐衡裵志恩
Owner AMOREPACIFIC CORP