Codonopsis glucan with immunoregulatory effect, its preparation method and application
A technology of immune regulation and dextran, which is applied in the fields of application, function of food ingredients, allergic diseases, etc., and can solve the problem that there is no relevant report on the immune regulation of Codonopsis dextran
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[0064] Specific embodiments of the present invention will be described in detail below.
[0065] 1. The preparation of Codonopsis glucan with immunomodulatory effect is as follows:
[0066] (1) Codonopsis polysaccharide extraction
[0067] Codonopsis pilosula is soaked for 30 minutes according to the material-to-liquid ratio of 1:10~1:20, heated and refluxed for 3 times, each time for 1.5 hours, and the three extracts are combined.
[0068] (2), preparation of Codonopsis polysaccharide
[0069] Codonopsis polysaccharide extract was concentrated under reduced pressure to 1 / 4~1 / 3 volume, precipitated with 60%~80% ethanol, centrifuged at 1000 rpm for 15min, removed the supernatant, and precipitated with ether, ethyl acetate, acetone, anhydrous Washed with ethanol three times in sequence, and freeze-dried to obtain a polysaccharide sample of Codonopsis pilosula.
[0070] (3) Ultrafiltration separation of Codonopsis polysaccharide
[0071] Dissolve Codonopsis polysaccharide in ...
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