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Application of CETP detection reagent in preparation of lung cancer screening kit

A kit and reagent technology, applied in the field of in vitro diagnostic reagents, can solve problems such as CETP protein that has not yet been seen, and achieve good application prospects, effective screening, and low patient harm

Pending Publication Date: 2021-02-26
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no prior art related to CETP protein and lung cancer

Method used

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  • Application of CETP detection reagent in preparation of lung cancer screening kit
  • Application of CETP detection reagent in preparation of lung cancer screening kit
  • Application of CETP detection reagent in preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The relationship between CETP and lung cancer in embodiment 1 plasma

[0026] 1. Clinical data

[0027] Select 40 cases of lung cancer patients and 40 cases of healthy controls. The basic information is as follows:

[0028] Basic Information lung cancer patients healthy control number of people 40 40 age 58.8±11.5 50.1±14.3 male ratio 33(82.5％) 17(44.7％)

[0029] 2. Detection principle

[0030] Same as the basic enzyme-linked immunosorbent assay (ELISA) detection method.

[0031] CETP protein CETP antibody (specifically recognizes the ASYPDITGEK amino acid sequence of CETP protein (SEQ ID NO.1)) is immobilized on the protein chip. After incubation with plasma exosome protein, if there is CETP protein in the exosome protein, the CETP protein It will bind; wash to remove unbound antibodies and other proteins, then use fluorescently labeled CETP antibody to bind to CETP protein for detection, and read the signal through a fluoresce...

Embodiment 2

[0051] Embodiment 2 The composition of detection kit of the present invention and its application method

[0052] 1. Kit composition

[0053] Detection kit (14 persons):

[0054]

[0055]

[0056] The reagents for preparing plasma exosomal proteins need to be prepared by themselves, which can be commercially available plasma exosomal protein extraction kits.

[0057] 2. How to use the kit

[0058] Same as the third part of Example 1.

[0059]The kit of the present invention can detect the risk of lung cancer in the population to be tested by detecting the level of CETP in plasma exosomes: if the CETP level is high (relative to healthy controls), the risk of lung cancer is high, and if CETP Low levels are associated with a low risk of lung cancer. It can be used for auxiliary diagnosis of clinical lung cancer, and provide effective basis for patients to take relevant treatment measures or decision-making, and has a good clinical application prospect.

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Abstract

The invention relates to the field of in-vitro diagnostic reagents, in particular to application of a CETP detection reagent in preparation of a lung cancer screening kit. What is found for the firsttime is that the level of CETP protein in plasma exosomes of lung cancer patients is significantly higher than that of CETP protein in healthy people. The reagent for detecting the CETP protein is used for preparing the lung cancer screening kit, and effective screening of lung cancer can be achieved.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of CETP detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cancer are very impo...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57423G01N33/57496
Inventor 田攀文李亚伦韩青兵姚梦琳张立
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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