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Gene conserved sequence, primer probe combination, kit and application for detecting neocoronavirus

A technology of conservative sequences and primer probes, which is applied in the field of biological and medical nucleic acid detection, can solve the problems of inseparable price and special equipment, time-consuming and other problems, and achieve fast and accurate detection results, ensuring accuracy and good specificity

Active Publication Date: 2021-03-09
SHANGHAI BIOGERM MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most laboratories use the PCR method to detect the new coronavirus, which has good sensitivity and specificity, but it takes a long time, and it is inseparable from expensive special equipment, so it is difficult to popularize it in grassroots inspection institutions.

Method used

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  • Gene conserved sequence, primer probe combination, kit and application for detecting neocoronavirus
  • Gene conserved sequence, primer probe combination, kit and application for detecting neocoronavirus
  • Gene conserved sequence, primer probe combination, kit and application for detecting neocoronavirus

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Embodiment Construction

[0085] A conservative sequence for detecting the new coronavirus gene, wherein the cDNA sequence includes: a conservative sequence in the target N gene and a conservative sequence in the target ORF1ab gene as the target sequence for detection, which is used to detect the new coronavirus, by further selecting the above conservative A segment of the N gene and ORF1ab gene conservative sequence in the sequence is used as the target sequence, and corresponding primer probes and internal reference primer probes are designed for the corresponding target sequences. The principles of primer design are as follows:

[0086] 1) The length of the RPA primer is 27-35 bases, and the GC content is between 40%-60%, so as to avoid the formation of secondary structure and hairpin structure;

[0087] 2) Special sequences such as long strings of polypurine or polypyrimidine should be avoided in the primers;

[0088] 3) The first 3-5 nucleotides at the 5' end should avoid polyguanine, preferably c...

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Abstract

The invention provides a gene conserved sequence, a primer probe, a kit and application for detecting neocoronavirus, and belongs to a molecular biological nucleic acid detection technology. In orderto solve the technical problems of better specific detection of the neocoronavirus, sensitivity during detection and the like, the invention provides the gene conserved sequence, the primer probe, thekit and the application for detecting an ORF1ab / N gene of the neocoronavirus, and belongs to the molecular biological nucleic acid detection technology. The invention aims to improve the specificity,the sensitivity and the detection efficiency of neocoronavirus detection, so that the whole detection process is simpler, more convenient, quicker and more efficient.

Description

technical field [0001] The present invention relates to the field of biological and medical nucleic acid detection, in particular to a gene conservation sequence, primer-probe combination, kit and application for detecting the new coronavirus. Background technique [0002] On January 12, 2020, the World Health Organization named the new coronavirus, namely "2019-nCoV". The virus can be transmitted by respiratory droplets or by close contact; the population is generally susceptible, and the elderly and those with underlying diseases are prone to develop severe infections. [0003] Common signs after coronavirus infection are respiratory symptoms, fever, cough, shortness of breath, and dyspnea. In more severe cases, infection can lead to pneumonia, severe acute respiratory syndrome, kidney failure, and even death. However, due to relatively hidden factors such as the lack of obvious initial symptoms and the incubation period of up to 14 days, patients and carriers of the new...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12N15/50C12N15/11C12R1/93
CPCC12Q1/701C12Q1/6844C07K14/005C12Q2600/166C12N2770/20022C12Q2521/107C12Q2521/507C12Q2522/101C12Q2531/119C12Q2545/101
Inventor 李沛李春燕朱兆奎杜沙沙
Owner SHANGHAI BIOGERM MEDICAL TECH CO LTD
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