Method for simultaneously preparing high-purity geniposide and gardenia yellow by one-step two-phase extraction

Abstract

The invention relates to a method for simultaneously preparing geniposide and gardenia yellow with high purity and high recovery rate through two-phase extraction. The method is to extract gardenia powder with ultrasonic-assisted food-grade solvent to obtain the primary extract, and then add salt, isopropanol or acetonitrile solution to form a two-phase aqueous system. After sufficient extraction, simple centrifugation can realize geniposide, Gardenia yellow is separated with high selectivity, and the purity of the lower phase geniposide can reach 90.58%, and the recovery rate can reach 95.72%; the purity of the upper phase gardenia yellow can reach 96.84%, and the recovery rate can reach 90.74%. The invention has the advantages of simple process, no need to add additional chelating agent, avoids the disadvantages of using a large amount of organic solvents in the traditional extraction method, is green and efficient, economical and time-saving, realizes one-step separation to obtain geniposide and gardenia yellow, and does not need to use expensive equipment , easy for industrial production.

Description

Method for preparing high-purity gardeniside and gardenia yellow simultaneously by one-step aqueous two-phase extraction (1) Technical field The present invention relates to a kind of method for preparing high-purity gardeniside and gardenia yellow simultaneously by one-step two-phase extraction. (2) Background technology Gardenia is Rubiaceae (Rubiaceae) Gardenia (Gardenia) plant, is the first batch of medicinal food promulgated by the Ministry of Health Dual-use resource. Gardenia is an evergreen shrub that is widely distributed in subtropical regions. There are about 250 species in the world. In my country, there are mainly gardenias. (Gardenia jasminoides Ellis), Spoon-leaf Gardenia, Narrow-leaf Gardenia and Hainan Gardenia 4 varieties, distributed in Zhejiang and Jiangxi West, Anhui, Taiwan and other regions. Astragaloside and gardenia yellow are the two most typical active components of Gardenia, accounting for the 12%, 15% or more. Gardenia yellow is widely...

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Problems solved by technology

The operation process of the above method is complicated, repeated extraction is required, and the assistance of auxiliary agents is required, and it is difficult to achieve simultaneous and efficient separation of geniposide and gardenia yellow and obtain the effect of high recovery rate

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