Culture medium for separating thraustochytrium limacinum and purification method of thraustochytrium limacinum

A technology for thraustochytrid and culture medium, which is applied in the field of marine microorganisms, can solve the problems of poor oxidation stability, heavy metal pollution, seasonal changes in oil components, etc., and achieves the effects of convenient operation, simple components and low cost.

Inactive Publication Date: 2021-03-12
TIANJIN UNIV MARINE TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fish oil has always been the traditional source of DHA. However, due to the high production cost of fish oil, seasonal changes in oil composition, scarcity of fish resources, heavy metal pollution, and poor oxidation stability, it is a healthy method to synthesize DHA through Thraustochytrium. , economical and environmentally friendly

Method used

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  • Culture medium for separating thraustochytrium limacinum and purification method of thraustochytrium limacinum
  • Culture medium for separating thraustochytrium limacinum and purification method of thraustochytrium limacinum
  • Culture medium for separating thraustochytrium limacinum and purification method of thraustochytrium limacinum

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Effect test

Embodiment 1

[0019] The seawater used in the present invention is collected from a bathing beach near Aoshan Bay, Qingdao, Shandong, and the collection time is October 1, 2018, using a culture medium for isolating Thraustochytrium to screen and cultivate:

[0020] (1) Preparation of culture medium for the isolation of Thraustochytrium: Glucose 5g, Peptone 2g, Yeast extract powder 0.5, sodium glutamate 0.5, corn steep liquor 1g, Agar 20g, sea salt 20g, natural pH, add ultrapure water to volume to 990ml, stir evenly with a glass rod, ultrasonicate for 5-10min, place in an autoclave at 115-121°C, and sterilize for 15-21min;

[0021] (2) Preparation of antibiotic mixture used for isolation and purification of Thraustochytrium culture medium: Weigh penicillin G 1g, Streptomycin 1.5g, Rifampicin 50mg, Nystatin 5mg, then add 10ml of ultrapure water, mix well and then shake with a shaker Until the mixed solution is completely dissolved, the antibiotic mixed solution needs to be prepared immediatel...

Embodiment 2

[0026] The seawater and rotten seaweed used in the present invention were collected from a wetland park near Jiaozhou Bay in the Yellow Sea, and the collection time was October 1, 2018. A culture medium for separating Thraustochytrium was used for screening and culturing:

[0027] (1) Preparation of medium for the isolation of Thraustochytrium: Glucose 1g, Peptone 0.5g, Yeast extract powder 2g, sodium glutamate 0.5g, corn steep liquor 0.5g, Agar 15g, sea salt 30g, natural pH after adding ultrapure water Make up to 990ml, stir evenly with a glass rod, sonicate for 5-10min, place in an autoclave at 115-121°C, and sterilize for 15-21min;

[0028](2) Preparation of antibiotic mixture used for isolation and purification of Thraustochytrium culture medium: Weigh 0.5g of penicillin G, 0.5g of Streptomycin, 100mg of rifampicin, 15mg of Nystatin, then add 10ml of ultrapure water, mix well and shake Shake until the mixture is completely dissolved, and the antibiotic mixture should be pr...

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Abstract

The invention relates to a culture medium for separating thraustochytrium limacinum and a purification method thereof. The culture medium for separating thraustochytrium limacinum is prepared from 1-5g of Glucose, 0.5-2 g of Peptone, 0.5-2 g of Yeaast exact power, 0.5-1 g of sodium glutamate, 0.5-1 g of corn steep liquor, 15-20g of Aar, 20-30g of sea salt, 0.5-1 g of penicillin G, 0.5-1.5 g of Streptomycin, 50-100 mg of rifampicin, 5-20 mg of Nystatin, and 1L of H2O, then steps of strain isolation, morphological observation, 18S rRNA sequencing are carried out; according to the method, thraustochytrium limacinum can be directly separated from rotten sea grass in marine water and wetland, the culture medium is simple in component, low in cost and convenient to operate, and the method can beused as a separation culture method of thraustochytrium limacinum for laboratories.

Description

technical field [0001] The invention relates to the field of marine microorganisms, in particular to a thraustochytrium separation medium and a separation and purification method thereof. Background technique [0002] Thraustochytrids are single-celled fungal heterotrophic protists in the ocean, widely distributed in marine environments such as oceans, wetlands, saltwater lakes, and seabed sediments. Currently Thraustochytrids are classified into: Stramenopila, Heterokonta, Labyrinthulomycetes, Thraustochytridiales, Thraustochytriidae ). Thraustochytrium not only exists widely in marine ecosystems, but also plays an important role in many fields. As a decomposer, any material in a state of decay in the ocean (including decaying animal and plant remains, marine animal feces, etc.) is a potential source of nutrition; Thraustochytrium has the ability to independently planktonic in the marine environment , but they are often used as parasitic microorganisms, parasitic in seaw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12R1/645
CPCC12N1/14C12N1/02
Inventor 汪光义温帅叶会科何艺科白默涵
Owner TIANJIN UNIV MARINE TECH RES INST
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