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Freeze-drying reagent of PCR premix liquid

A premix, freeze-drying technology, applied in the field of molecular biology, can solve the problem of large gap in amplification efficiency, achieve stable fluorescence value, solve the problem of product performance instability, and amplify fluorescence value.

Pending Publication Date: 2021-03-12
GUANGDONG FAPON BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The third object of the present invention is to provide a lyophilized PCR premix to alleviate the problems of the prior art that the lyophilized PCR premix has a large gap in amplification efficiency compared with that before lyophilization.

Method used

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  • Freeze-drying reagent of PCR premix liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] A stable formulation for lyophilized PCR master mix consisting of hydrogenated maltose 1w / v%. No. 1-3 freeze-dried products have poor shape, CT value is 27.2-28.0, and fluorescence value is 12%-15% lower than liquid state.

Embodiment 2

[0071] A stable formulation for lyophilized PCR master mix consisting of hydrogenated maltose 12w / v%. Freeze-dried products No. 1-3 were formed well, with CT value of 27.9-28.8, fluorescence value decreased by 16%-18% compared with liquid state, and activity decreased by 19%-21% after 1-month assessment at 37°C.

Embodiment 3

[0073] A stable formulation for lyophilized PCR master mix consisting of hydrogenated maltose 3w / v%. Freeze-dried products No. 1-3 have good shape, CT value is 25.8-26.3, fluorescence value is 6%-8% lower than that of liquid state, and activity is 20%-23% lower than that of liquid state after 1-month assessment at 37°C.

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Abstract

The invention relates to the field of molecular biology, and particularly provides a freeze-drying reagent of PCR premix liquid. A stable prescription is added into the PCR premix liquid to realize freeze-drying of the PCR premix liquid, the stable prescription is simple in component, a freeze-dried finished product is good in forming and long in stable period, and the stable prescription does notinfluence the PCR amplification efficiency.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a reagent for freeze-dried PCR premix. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction, PCR) is a molecular biology technique used to amplify specific gene fragments. At present, this technique has been widely used all over the world because of its high sensitivity and excellent performance. specificity, and has the advantages of simple and fast operation, and has played a pivotal role in biological and medical research. [0003] Currently, most of the PCR reagents used for genetic testing in the market are liquid reagents, and the components required for amplification usually need to be divided into several tubes, and storage at room temperature is not stable, so cryopreservation and transportation are required. Storage and transportation bring great inconvenience, and the validity period of reagents is short, which increases the risk of conta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6876
CPCC12Q1/6876
Inventor 周猛冲章瑞程林钰琼黄杏帆范国来
Owner GUANGDONG FAPON BIOTECH CO LTD