Supercharge Your Innovation With Domain-Expert AI Agents!

Efficient extraction method of total DNA of marine shellfish biological sediments

An extraction method and sediment technology, applied in the field of marine shellfish biological sediment research, can solve the problems of ineffective removal of humus, phenolic substances, imperfect technical methods, etc.

Active Publication Date: 2021-03-16
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research methods for DNA extraction in marine shellfish sediments mainly include SDS high-salt extraction method and kit extraction, but these methods have not effectively removed humus, phenolic substances and other pollutants, and the technical methods are not yet perfect. , therefore, there is still a lot of room for exploration regarding methods for the extraction of total microbial DNA from marine shellfish biosediments, especially in terms of sediment pretreatment.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Efficient extraction method of total DNA of marine shellfish biological sediments
  • Efficient extraction method of total DNA of marine shellfish biological sediments
  • Efficient extraction method of total DNA of marine shellfish biological sediments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take the same sediment and follow the steps below to perform 3 sets of parallel operations to obtain 3 sets of parallel samples.

[0039] (1) Washing of samples:

[0040] (1.1) Take 400mg of sediment sample in a 2ml centrifuge tube, add 600μl of washing solution 1 and vortex for 2min, 12000

[0041] Centrifuge at rpm for 7min and discard the supernatant.

[0042] (1.2) Add 600 μl of washing solution 2, vortex for 2 minutes, centrifuge at 12000 rpm for 7 minutes, and discard the supernatant.

[0043] (1.3) Add 600 μl of washing solution 3, vortex for 2 minutes, centrifuge at 12000 rpm for 7 minutes, and discard the supernatant.

[0044] (2) Bacterial cell lysis:

[0045] (2.1) Add 400 μl of extraction buffer to the sediment treated in step (1.3), and mix evenly on a mini-stirrer at 5000 rpm for 10 s.

[0046] (2.2) Immerse the centrifuge tube in liquid nitrogen until the sediment mixture is completely frozen, then place it in a water bath at 55°C to thaw, and repeat th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of marine shellfish biological sediment research, and relates to an efficient extraction method of total DNA of marine shellfish biological sediments. Theefficient extraction method comprises two steps of pretreatment of a marine shellfish biological sediment sample and extraction of the total DNA of the sample, wherein the pretreatment of the sample is the key step; and three types of washing solutions of different components are used in sequence to carry out pretreatment on the sediment sample to remove biological pollutants and impurities including phenolic substances, humus and the like. an extraction buffer is added into the impurity-removed sample, the steps of cell lysis, extraction and the like are carried out, and DNA precipitates arecollected. According to the efficient extraction method, on the principle of biochemistry, molecular biology and microbiology, the high-quality microorganism DNA in the marine shellfish biological sediments is extracted, so that the subsequent accurate and comprehensive molecular biology research is facilitated.

Description

technical field [0001] The invention belongs to the technical field of marine shellfish biological sediment research, and relates to a method for efficiently extracting total DNA of marine shellfish biological sediment, which mainly includes two steps of pretreatment of marine shellfish biological sediment samples and total DNA extraction of samples. Background technique [0002] The marine ecological environment is an important part of the natural ecosystem, and the microbial diversity in seabed sediments is crucial to maintaining the balance of marine ecology. For the study of microbial diversity in marine sediments, DNA obtained by conventional microbial DNA extraction methods (such as: phenol-chloroform method, SDS high-salt extraction method, kit method, etc.) Follow-up data analysis. [0003] Since marine shellfish sediments contain a large amount of impurities such as humus, phenolic substances, and heavy metals, the structure of these pollutants is relatively comple...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 朱玲孙安琪李蒙
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com