Lung cancer methylation and colorectal cancer methylation judgment devices

Abstract

The invention provides lung cancer methylation and colorectal cancer methylation judgment devices. The lung cancer methylation judgment device comprises an acquisition module, a difference calculationmodule, a methylation pre-judgment module and a methylation judgment module, wherein the acquisition module is used for acquiring a first Ct value of a fluorescence channel internal reference, a second Ct value of an RASSF1A gene and a third Ct value of an SHOX2 gene; the difference calculation module is used for calculating a first difference between the second Ct value and the first Ct value and a second difference between the third Ct value and the first Ct value; the methylation pre-judgment module is used for pre-judging whether methylation is positive or non-positive according to a result acquired by the acquisition module and a result calculated by the difference calculation module; and the methylation judgment module is used for judging whether methylation is positive or negativeaccording to a pre-judged methylation positive or non-positive result and the first Ct value. The lung cancer methylation and colorectal cancer methylation judgment devices are high in diagnosis sensitivity and reliability for early lung cancer and colorectal cancer, simple to operate, easy to interpret, low in cost and wide in applicability.

Description

technical field [0001] The invention relates to the field of gene diagnosis, in particular to a device for judging methylation of lung cancer and methylation of colorectal cancer. Background technique [0002] Lung cancer has become one of the main causes of human cancer death. Lung cancer is the cancer with the highest incidence rate in China, and its mortality rate is also increasing rapidly. The incidence and mortality of lung cancer are the highest among all tumors. However, the early diagnosis of lung cancer is not the highest. In the United States, breast tumors and prostate tumors have early diagnosis. The 5-year survival rate of tumors is 89 and 99%, respectively), while the 5-year survival rate of lung cancer is only 15%. [0003] In clinical practice, early diagnosis of lung cancer has always been difficult, and early detection of cancer is very important for the effective treatment of cancer patients. At present, the diagnosis of cancer is mainly based on clin...

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Problems solved by technology

[0007] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a device for judging methylation of lung cancer and colorectal cancer, which is used to solve the problem that the methylation of lung cancer and colorectal cancer cannot be performed simply, quickly and conveniently in the prior art. Problems in the judgment of methylation in colorectal cancer

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