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Kit and system for detecting mycobacterium chelonae

A technology of mycobacteria and kits, which is applied in the system field of detection of Mycobacterium tortoise, can solve the problems of low resolution and the like

Active Publication Date: 2021-03-23
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of this disclosure is to solve the problem of low resolution existing in the existing NTM detection technology, and to provide a kit and system capable of accurately detecting Mycobacterium chelonis

Method used

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  • Kit and system for detecting mycobacterium chelonae

Examples

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Embodiment 1

[0044] This example is used to illustrate the method for determining the biomarkers provided by the present disclosure.

[0045] 81 NTM strains with complete genomes were collected as a discovery group, including 7 strains of Mycobacterium kansasii, 27 strains of Mycobacterium avium, 6 strains of Mycobacterium intracellulare, 5 strains of Mycobacterium chelonis and 36 strains of Mycobacterium abscessus bacilli.

[0046]In addition, 384 NTM strains with original sequencing reads were collected as the first verification group, including 14 strains of Mycobacterium kansasii, 111 strains of Mycobacterium avium, 26 strains of Mycobacterium intracellulare, 33 strains of Mycobacterium chelonis and 200 strains of Mycobacterium abscessus.

[0047] Comparative genomics analysis was performed on the genome data of the discovery group, and the distinguishing genes and distinguishing SNPs sites in the genomes of the five strains of Mycobacterium chelonis that were different from those of ...

Embodiment 2

[0050] This example is used to verify the detection effect of the biomarkers provided by the present disclosure on Mycobacterium chelonae.

[0051] 164 NTM strains with original sequencing reads were collected as the second verification group, including 6 strains of Mycobacterium kansasii, 48 strains of Mycobacterium avium, 11 strains of Mycobacterium intracellulare, 14 strains of Mycobacterium chelonis and 85 strains of Mycobacterium abscessus.

[0052] The nucleic acid extraction kit is used to extract the total nucleic acid of the above five kinds of mycobacteria respectively, to obtain the total nucleic acid of each mycobacterium. Next-generation sequencing is then performed on the total nucleic acid of each mycobacterium obtained above to obtain the total nucleic acid sequence of each mycobacterium.

[0053] Based on at least part of the molecular markers determined in Example 1, it is judged whether there is a corresponding molecular marker sequence in the total nucleic...

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Abstract

The invention relates to a kit and system for detecting mycobacterium chelonae. The kit comprises a reagent for detecting a molecular marker, wherein the molecular marker comprises at least one of a yjcH gene, an mpr gene, an rrl C2638T locus and an rrl G2654A locus. By identifying the molecular marker, accurate detection of the mycobacterium chelonae can be realized.

Description

technical field [0001] The present disclosure relates to the field of biotechnology, in particular, to a kit for the detection of Mycobacterium chelonis, the use of a reagent for detecting molecular markers in the preparation of a kit for the detection of Mycobacterium A system for the detection of mycobacteria. Background technique [0002] Non-tuberculous Mycobacterium (NTM) refers to mycobacteria other than Mycobacterium tuberculosis (Mtb) complex and Mycobacterium leprae, which widely exist in the natural environment . About one-third of NTM can cause animal or human infection, such as Mycobacterium kansasii, Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium abscessus and Mycobacterium chelonis. [0003] NTM infection is common in patients with structural lung diseases. After infecting the human body, NTM often invades the lung tissue of the patient, leading to progressive destruction of the bronchi and alveolar tissue, causing symptoms similar to tuberc...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/04C12N15/11G16B20/30
CPCC12Q1/689G16B20/30C12Q2600/156
Inventor 贾鑫淼杨启文陈非李翠丹
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI