Straw decomposing bacterial agent in cold regions, its preparation method and application

A production method and antibacterial agent technology, applied in the field of agricultural preparations, can solve the problems of high manpower and economic costs

Active Publication Date: 2021-09-07
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the actual use of this bacterial agent, the human and economic costs such as transportation and spraying are relatively high.

Method used

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  • Straw decomposing bacterial agent in cold regions, its preparation method and application
  • Straw decomposing bacterial agent in cold regions, its preparation method and application
  • Straw decomposing bacterial agent in cold regions, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The invention relates to a straw decomposing bacterial agent in cold regions, which consists of microcapsules of low-temperature bacterial flora and bacteria powder of normal-temperature lignocellulose-decomposing flora.

[0049] (1) Production of low-temperature flora microcapsules:

[0050] ①Preparation of improved low-temperature bacterial culture medium: the preparation contains glucose 15g / L, K 2 HPO 4 1.0 g / L, KH 2 PO 4 1.0g / L, peptone 6g / L, beef extract 6g / L, yeast powder 3g / L, MgSO 4 ·7H 2 O0.1g / L, sodium acetate 2g / L, diammonium hydrogen citrate 2g / L, MnSO 4 ·H 2 O 0.05g / L, Tween 801mL / L improved low-temperature bacterial culture medium. Divide into 500mL blue cap bottles, each bottle contains 350mL. Store in a 4°C refrigerator for later use.

[0051] ②Floral culture: Inoculate Lactobacillus plantarum, Lactococcus lactis subsp. lactis, Lactococcus, Arthrobacter globosa and Rhodococcus into the improved low-temperature culture medium. After culturing a...

Embodiment 2

[0061] The invention relates to a straw decomposing bacterial agent in cold regions, which is composed of: freeze-dried powder of low-temperature bacterial flora and bacterial powder of normal-temperature lignocellulose-decomposing bacterial flora.

[0062] (1) Production of low-temperature flora freeze-dried powder:

[0063] ①Preparation of improved low-temperature bacterial culture medium: the preparation contains glucose 15g / L, K 2 HPO 4 1.0 g / L, KH 2 PO 4 1.0g / L, peptone 6g / L, beef extract 6g / L, yeast powder 3g / L, MgSO 4 ·7H 2 O0.1g / L, sodium acetate 2g / L, diammonium hydrogen citrate 2g / L, MnSO 4 ·H 2 O 0.05g / L, Tween 801mL / L improved low-temperature bacterial culture medium. Divide into 500mL blue cap bottles, each bottle contains 350mL. Store in a 4°C refrigerator for later use.

[0064] ②Floral culture: Inoculate Lactobacillus plantarum, Lactococcus lactis subsp. lactis, Lactococcus, Arthrobacter globosa and Rhodococcus into the improved low-temperature culture...

Embodiment 3

[0070] The bacterial agent prepared in Example 1 was used as the experimental group, and was simultaneously inoculated with four commercially available straw-decomposing bacterial agents CK1, CK2, CK3, and CK4 on a medium with rice straw as the only carbon source, and fermented at 28°C for 15 days , to determine the weight-reducing ability of rice straw. Under different treatments, the analysis of rice straw weight loss is as follows: figure 1 shown. Depend on figure 1 It can be seen that the straw weight loss under the treatment of the bacterial agent, CK1, CK2, CK3, and CK4 was 0.72g, 0.67g, 0.63g, 0.58g, and 0.54g, and the weight loss rates were 44.2%, 41.10%, and 38.65% respectively. %, 35.58%, 33.12%. It shows that the straw transformation ability of this inoculum agent under the same conditions is stronger than that of the four commercially available inoculum agents used in the experiment.

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Abstract

The invention provides a cold region straw decomposing fungus and a preparation method and application thereof, belonging to the technical field of agricultural preparations. The cold region straw decomposing bacteria agent of the invention includes low temperature bacteria group and normal temperature bacteria group; the low temperature bacteria group includes Lactobacillus plantarum with a cell number content of 20-28%, and Lactococcus lactis subsp. lactis with a cell content of 17-25%. , 14-22% of Lactococcus, 11-22% of Arthrobacter sphaericus and 9-18% of Rhodococcus; the normal temperature flora is lignocellulolytic flora. The product provided by the invention can start fermentation under low temperature conditions, promote the decomposition of straw under low temperature conditions, improve the in-situ decomposition efficiency of straw in cultivated land, and solve the problems of dead seedlings and yield reduction caused by severe decomposition of straw in paddy fields in cold regions of my country in summer.

Description

technical field [0001] The invention belongs to the technical field of agricultural preparations, and in particular relates to a straw decomposing bacterial agent in cold regions, a preparation method and application thereof. Background technique [0002] Straw is the most productive renewable resource in the world. According to incomplete statistics, the annual output of straw in the world is 85 billion tons, and the annual output of crop straw in my country alone reaches 850 million tons. Lignocellulose in straw is combined by cellulose, hemicellulose, lignin, ash and other substances, which makes cellulose difficult to be hydrolyzed, so it is compared to "concrete". There is a huge amount of straw in the main grain-producing areas of Northeast China. With the sharp decline in the marginal effect of the investment ratio of cultivated land, the degraded black soil urgently needs to be fertilized, and there is an urgent need for straw returning to the field to improve the e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/16C12N1/14C12N1/04C12N11/10C12N11/04C05F11/08C05F17/20C12R1/25C12R1/06C12R1/01C12R1/69C12R1/80C12R1/885C12R1/125C12R1/145C12R1/085C12R1/865C12R1/84
CPCC05F11/08C12N1/04C12N1/14C12N1/16C12N1/18C12N1/20C12N11/04C12N11/10C05F17/20Y02W30/40
Inventor 刘长莉
Owner NORTHEAST FORESTRY UNIVERSITY
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