SNP molecular marker method and primers used in screening goose egg quality

Abstract

The invention discloses an SNP molecular marker method and primers used in screening goose egg quality, and relates to the field of molecular biology. In order to achieve the purpose, according to themethod and the primers, by utilizing a nucleic acid time-of-flight mass spectrometry technology, 32 SNP molecular markers of seven egg qualities of Sichuan white geese can be screened at one time. Specifically, the invention discloses the SNP molecular marker method for screening multiple Sichuan white goose egg qualities based on a nucleic acid time-of-flight mass spectrometry method and primersused by the SNP molecular marker method for the 32 molecular markers of the following seven egg qualities including egg weight, egg specific gravity, egg relative density, eggshell strength, eggshellthickness, eggshell weight and yolk weight. The primers comprise PCR upstream and downstream amplification primers and a single base extension primer probe sequence. Individuals with target traits can be quickly and accurately bred in the gosling stage, the breeding efficiency is greatly improved, and the breeding cost is reduced.

Description

technical field [0001] The invention relates to a SNP molecular marker method and primers for screening the quality of goose eggs, and mainly relates to the field of molecular biology. Background technique [0002] Sichuan white goose is an excellent local breed in China, with the characteristics of strong stress resistance, good adaptability, resistance to rough feeding, strong disease resistance and resistance to rough feeding, etc. It provides meat, eggs, down, fatty liver, etc. to consumers. It is widely raised throughout China and further used for the improvement and creation of other varieties. Egg quality traits are not only important economic traits, but also complex quantitative traits, all of which are affected by heredity, nutrition, feeding methods, and external environment. Using biological and other methods to conduct research, molecular markers such as duck body size and egg shell color have been discovered and widely used in molecular marker-assisted selecti...

AI Technical Summary

Problems solved by technology

However, the breeding work is limited by practical difficulties and the characteristics of seasonal breeding of geese. It is difficult to systematically and effectively select the quality of goose eggs, and it also lacks the appeal to consumers and the market, which limits the industrial development of Sichuan white geese.

Images