Molecular marking method for detecting goose hatching egg qualification rate

A technology of molecular markers and pass rate, applied in the field of molecular biology, can solve the problems of high cost and difficult breeding, and achieve the effect of improving efficiency and reducing cost

Active Publication Date: 2021-03-02
CHONGQING ACAD OF ANIMAL SCI
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AI Technical Summary

Problems solved by technology

The traditional method consumes a lot of manpower and material resources to detect the qualified rate of breeding eggs, and the cost is relatively high; and it is difficult to select corresponding female geese for the qualified rate of egg production of geese

Method used

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  • Molecular marking method for detecting goose hatching egg qualification rate
  • Molecular marking method for detecting goose hatching egg qualification rate
  • Molecular marking method for detecting goose hatching egg qualification rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Genome-wide association analysis of qualified rate of breeding eggs

[0049] Genome-wide association analysis:

[0050] At the age of 20 weeks, Sichuan white geese collected 2 mL of blood from the wing veins using vacuum blood collection tubes containing sodium heparin, extracted blood genome DNA using a blood genome extraction kit (Beijing Tiangen Company, DP332), and measured DNA using electrophoresis experiments and a NanoDrop2000 spectrophotometer The concentration and integrity of the stock solution were dissolved in TE solution and stored at -20°C for later use. The genomic DNA was transported by dry ice to Beijing Novogene Technology Co., Ltd. for whole genome resequencing.

[0051] At the age of 20 weeks, Sichuan white geese collected 2 mL of blood from the wing veins using vacuum blood collection tubes containing sodium heparin, extracted blood genome DNA using a blood genome extraction kit (Beijing Tiangen Company, DP332), and measured DNA using electroph...

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Abstract

The invention relates to the technical field of SNP (Single Nucleotide Polymorphism) molecular markers and provides a molecular marker method for detecting goose hatching egg qualification rate. The method comprises the following steps of comparing whole genome resequencing data to a goose reference genome sequence to obtain whole genome SNP sites, and obtaining seven candidate SNP molecular markers of goose hatching egg qualification rate characters by a whole genome correlation analysis method; and detecting the polymorphism of the seven SNP molecular markers by using a time-of-flight mass spectrometry method and counting the genotype frequency of the seven SNP molecular markers. And one haplotype module is also remarkably related to the egg laying percent of pass. The polymorphism SNP molecular marker and the primer pair comprise at least one of seven SNP molecular markers. According to the universal polymorphic SNP molecular marker for the Sichuan white geese obtained by the screening method, seven effective SNP molecular markers are provided, a database of the SNP molecular markers of the Sichuan white geese is supplemented, individuals with high or low hatching egg percent ofpass can be rapidly detected by utilizing the method, the breeding time and cost of traditional goose breeding are saved, and breeding efficiency is improved; reference is provided for development ofSichuan white goose variety resources and industrial development.

Description

technical field [0001] The invention relates to a molecular marker method for detecting the qualified rate of goose eggs, and mainly relates to the field of molecular biology. Background technique [0002] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) mainly refers to the polymorphism of DNA sequence caused by the variation of a single nucleotide in the genome series. It is the most common variation in the heritable variation of animal and plant genomes, and it exists widely in the genome. SNP has low mutation rate, high accuracy and stability, and is widely used as one of the molecular markers of animals and plants. It is widely used in locating disease target genes, individual screening in the process of animal and plant breeding, analysis of species evolution process and identification of kinship. [0003] my country is the country that raises the most geese, but due to various reasons, the development of goose industry is slow, especially the ben...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2533/101C12Q2521/525C12Q2565/627
Inventor 高广亮赵献芝王誉杰王丽辉吴睿张克山王海威王启贵卢茵谢友慧王珍李静尹春辉汪超
Owner CHONGQING ACAD OF ANIMAL SCI
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