Method for constructing rat model for proliferative diabetic retinopathy (PDR)

A technology of diabetic retina and construction method, which is applied in the field of rat model construction of proliferative diabetic retinopathy, can solve the problems of the impact of research results and large differences in pathophysiological processes, and achieve good repeatability, low cost, and promising application prospects broad effect

Pending Publication Date: 2021-04-06
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the pathophysiological process of the above-mentioned model after gene editing is quite different from that of human PDR, which will also have a certain impact on the research results

Method used

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  • Method for constructing rat model for proliferative diabetic retinopathy (PDR)
  • Method for constructing rat model for proliferative diabetic retinopathy (PDR)
  • Method for constructing rat model for proliferative diabetic retinopathy (PDR)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 ZDF rat model construction of proliferative diabetic retinopathy

[0035] Modeling method: 15 male obese ZDF rats (ZuckerDiabetic Fatty rats) aged 5-6 weeks and weighing 128.1±7.8g were selected, and the adaptation period was 1-2 weeks. Acclimate to the environment before the formal experiment. After entering the model period, feed them with Purina5008 feed for 2-6 months. Among them, the mass percentages of the main nutritional components in the Purina5008 feed formula are: protein 26.85%, fat 16.71%, carbohydrate 56.44%.

experiment example 1

[0039] 1. Reagents, instruments and appliances:

[0040] List of antibodies used in the experiment:

[0041] Table 1

[0042]

[0043] Instruments used in the experiment

[0044] Table 2

[0045]

[0046] The main computer software or data system used in the experiment

[0047] table 3

[0048]

[0049] 2. Detection method

[0050] In this experimental example, the ZDF rat model modeled in Example 1 was recorded as the experimental group (ZDF), and the Zucker lean rat model modeled in Comparative Example 1 was recorded as the control group (CON).

[0051] 1) weight

[0052] Measuring animals: all surviving rats in each group;

[0053] Measurement time: 1 time / week;

[0054] 2) Blood sugar (GLU)

[0055] Measuring animals: all surviving rats in each group;

[0056] Measurement time: 1 test during the adaptation period, 1 test per week during the model period;

[0057] Determination method: Fasting for at least 8 hours before sampling and testing, without wat...

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Abstract

The invention belongs to the technical field of diabetic animal model construction, and particularly relates to a method for constructing a ZDF (Zucker diabetic fatty) rat model for proliferative diabetic retinopathy (PDR). The method for constructing the model with PDR comprises the following steps: carrying out high-fat and high-sugar diet feeding on ZDF rats, and inducing hyperglycemia formation and dyslipidemia to obtain the rat model for PDR. Experiments find that the rat model obtained by the method can be successfully constructed, the obtained model has obviously increased blood sugar, abnormal lipid metabolism, retinal neovascularization indicated by fundus angiography FFA, vitreous opacity, retinal inner layer thinning indicated by OCT, retinal inner layer thinning indicated by pathology, remarkable reduction of ganglion cells and alteration of garland-like loss of the outer nuclear layer, the modeling time is short, and the application prospect is excellent.

Description

technical field [0001] The invention belongs to the technical field of diabetic animal model modeling, and in particular relates to a method for constructing a rat model of proliferative diabetic retinopathy. Background technique [0002] The global prevalence of diabetes and its complications continues to rise, posing a serious threat to human health. Among them, diabetic retinopathy (DR) is the most common ocular complication of diabetes and the most important cause of blindness in the working-age population worldwide. Since the pathogenesis of DR has not been fully elucidated, its prevention and treatment are still difficult. According to the severity of lesions, DR is divided into non-proliferative DR (NPDR) and proliferative DR (PDR). Microvascular abnormalities; retinal neovascularization is a hallmark feature of PDR. Diabetic macular edema, as the main cause of vision loss, can occur at any stage of NPDR or PDR. [0003] Current studies suggest that early retinal ...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A61K49/0008
Inventor 王钰娇岑小波殷宏玉张朝茂伍江波
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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