Method for constructing rat model for proliferative diabetic retinopathy (PDR)
A technology of diabetic retina and construction method, which is applied in the field of rat model construction of proliferative diabetic retinopathy, can solve the problems of the impact of research results and large differences in pathophysiological processes, and achieve good repeatability, low cost, and promising application prospects broad effect
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[0034] Example 1 ZDF rat model construction of proliferative diabetic retinopathy
[0035] Modeling method: 15 male obese ZDF rats (ZuckerDiabetic Fatty rats) aged 5-6 weeks and weighing 128.1±7.8g were selected, and the adaptation period was 1-2 weeks. Acclimate to the environment before the formal experiment. After entering the model period, feed them with Purina5008 feed for 2-6 months. Among them, the mass percentages of the main nutritional components in the Purina5008 feed formula are: protein 26.85%, fat 16.71%, carbohydrate 56.44%.
experiment example 1
[0039] 1. Reagents, instruments and appliances:
[0040] List of antibodies used in the experiment:
[0041] Table 1
[0042]
[0043] Instruments used in the experiment
[0044] Table 2
[0045]
[0046] The main computer software or data system used in the experiment
[0047] table 3
[0048]
[0049] 2. Detection method
[0050] In this experimental example, the ZDF rat model modeled in Example 1 was recorded as the experimental group (ZDF), and the Zucker lean rat model modeled in Comparative Example 1 was recorded as the control group (CON).
[0051] 1) weight
[0052] Measuring animals: all surviving rats in each group;
[0053] Measurement time: 1 time / week;
[0054] 2) Blood sugar (GLU)
[0055] Measuring animals: all surviving rats in each group;
[0056] Measurement time: 1 test during the adaptation period, 1 test per week during the model period;
[0057] Determination method: Fasting for at least 8 hours before sampling and testing, without wat...
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