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Method for culturing cells in pulsating flow mode and cell reaction instrument

A cell reactor and cell culture technology, which is applied in the field of pulsating flow culturing cells and cell reaction devices, can solve the problems of lack of methods and devices, insufficient nutrition of cells in the rear reactor, and restore liver function. Effect

Active Publication Date: 2021-04-06
山东壹瑞特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical problem that the cells in the back-stage reactor cannot get enough nutrition and lacks reasonable methods and equipment to solve the technical problems in the prior art, and provides a method for culturing cells in a pulsating flow mode and a cell reaction equipment

Method used

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  • Method for culturing cells in pulsating flow mode and cell reaction instrument
  • Method for culturing cells in pulsating flow mode and cell reaction instrument
  • Method for culturing cells in pulsating flow mode and cell reaction instrument

Examples

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Effect test

Embodiment 1

[0038]Example 1 A cell reaction device for culturing C3A cell lines in a pulsating flow mode

[0039] A cell reactor for cultivating cells in a pulsating flow mode, which includes a hollow fiber cell bioreactor 2, characterized in that the front end of the hollow fiber cell bioreactor 2 is connected to a liquid pump that ensures that liquid containing nutrients and oxygen flows into the cell reactor 1; The hollow fiber cell bioreactor 2 is connected to the back end to ensure periodic blocking of the outflow of liquid containing nutrients and oxygen to form a pulsating flow blocking structure 3; The entire cell reactor is connected or closed within a specific period through an intermittent structure Fluid pathways containing nutrients and oxygen.

[0040] The hollow fiber cell bioreactor 2 includes a columnar structure 21 for accommodating multiple hollow fiber tubes 22, and the two sides of the columnar structure 21 are provided with inlet pipes connecting the liquid containin...

Embodiment 2

[0044] Example 2 A cell reaction device for culturing C3A cell lines in a pulsating flow mode

[0045] A cell reactor for cultivating cells in a pulsating flow mode, which includes a hollow fiber cell bioreactor 2, characterized in that the front end of the hollow fiber cell bioreactor 2 is connected to ensure that the liquid containing nutrients and oxygen flows into the cell reactor, and the front end flows into the cell reactor. The pipeline is controlled by the liquid pump 1; the back end of the hollow fiber cell bioreactor 2 is connected to a blocking structure that periodically blocks the outflow of the liquid containing nutrients and oxygen to form a pulsating flow; through the intermittent structure, it is connected in a specific period or Close the passage of liquid containing nutrients and oxygen throughout the cell reactor.

[0046] The hollow fiber cell bioreactor 2 includes a columnar structure 21 for accommodating multiple hollow fiber tubes 22, and the two sides...

Embodiment 3

[0051] Example 3 A cell reaction device for culturing C3A cell lines in a pulsating flow mode

[0052] A cell reactor for cultivating cells in a pulsating flow mode, which includes a hollow fiber cell bioreactor 2, characterized in that the front end of the hollow fiber cell bioreactor 2 is connected to a liquid pump that ensures that liquid containing nutrients and oxygen flows into the cell reactor 1; The back end of the hollow fiber cell bioreactor 2 is connected to ensure periodic blocking of the outflow of liquid containing nutrients and oxygen to form a blocking structure that forms a pulsating flow; through the intermittent structure, the entire cell reactor is connected or closed in a specific period. Liquid pathways for nutrients and oxygen.

[0053] The hollow fiber cell bioreactor 2 includes a columnar structure 21 for accommodating multiple hollow fiber tubes 22, and the two sides of the columnar structure 21 are provided with inlet pipes connecting the liquid cont...

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Abstract

The invention discloses a cell reactor for culturing cells in a pulsating flow mode, which comprises a hollow fiber cell bioreactor, and is characterized in that the front end of the hollow fiber cell bioreactor is connected with a liquid pump for ensuring that liquid containing nutrient substances and oxygen flows into the cell reactor; the rear end of the hollow fiber cell bioreactor is connected with a blocking structure which ensures that the liquid containing nutrient substances and oxygen is periodically blocked from flowing out to form pulsating flow; and a liquid passage containing nutrient substances and oxygen of the whole cell reactor is communicated or closed in a specific period through an intermittent structure. The blocking structure comprises a pressure detection structure, a control structure and a clamping and closing structure, the value of the pressure detection structure is received through the control structure, opening and closing of the clamping and closing structure are adjusted in a feedback mode, pulsating flow can be well formed in the cell bioreactor through intermittent opening and closing, transmembrane movement of substances between fiber tubes in the fiber tubes is facilitated, and cell growth is facilitated.

Description

technical field [0001] The invention belongs to equipment in the fields of cell therapy, biomedicine, cell protein and exogenous factor production and treatment, and mainly relates to the manufacture and treatment of bioartificial liver and the production of other cell products. Specifically, the present invention relates to a method for the treatment of bioartificial liver for patients with liver failure, which is an important cell-based product that can significantly improve the therapeutic effect of in vitro bioartificial liver. A method for culturing cells in a pulsating flow mode and a cell reaction device thereof. Background technique [0002] The cell culture in the prior art will carry out three-dimensional cell culture in a structure containing many hollow fiber tubes. The cells grow outside the fiber lumen of the hollow fiber tubes. The permeable membrane structure, so the culture medium passes through the hollow fiber tube lumen as much as possible according to t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36C12M1/34C12M1/24C12M1/00C12N5/071
CPCC12M25/10C12M29/12C12M23/48C12M29/26C12M41/44C12M41/40C12N5/0671C12N2513/00
Inventor J·布拉泽顿D·贺阮海斌
Owner 山东壹瑞特生物科技有限公司
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