Method for directly inducing emergence of seedlings by utilizing pseudo-ginseng stems

Abstract

The invention discloses a method for directly inducing emergence of seedlings by utilizing pseudo-ginseng stems, and belongs to the technical field of pseudo-ginseng cultivation. The method comprises the following steps of taking a sterile culture dish, paving a layer of sterile filter paper on the culture dish, pouring some sterile water containing NAA and SA, and wetting the sterile filter paper; taking out pseudo-ginseng tissue culture seedlings, placing the pseudo-ginseng tissue culture seedlings on wet filter paper, cutting off leaves and roots, only reserving the stems, and cutting the stems into small sections of 1-2cm; and placing the stems into a new culture medium containing a corresponding formula, wherein for the formula of the culture medium, 0.5-1 mg / L of NAA and 0.1-0.7 mg / L of salicylic acid are added to an MS culture medium, and the MS culture medium is put into a tissue culture room for culture after film sealing. According to the method, the stems are adopted to directly induce emergence of the seedlings, the callus stage is avoided, and the emergence rate and success rate of tissue culture of pseudo-ginseng explants are remarkably increased.

Description

technical field [0001] The invention relates to a method for directly inducing seedling emergence by utilizing stem segments of Panax notoginseng, and belongs to the technical field of Panax notoginseng cultivation. Background technique [0002] Panax notoginseng (Burk.) F.H.Chen is a plant of the genus Panax notoginseng in the family Araliaceae, which belongs to traditional precious Chinese medicinal materials in my country. Panax notoginseng has the reputation of "gold does not change" and "sacred grass in the south"; because panax notoginseng contains active ingredients such as notoginseng saponins, it has the effects of dispelling blood stasis, stopping bleeding, reducing swelling and relieving pain. Its main active ingredient is saponin, and there is no artificial synthesis method at present; Panax notoginseng, a shade-loving plant, has very high requirements for growth conditions. It likes an environment that is warm in winter and cool in summer, and is afraid of sever...

AI Technical Summary

Problems solved by technology

[0003] There are only a few places in the world where Panax notoginseng can be planted, and Panax notoginseng has a long growth cycle and serious pests and diseases. Therefore, the supply of Panax notoginseng resources in the market is insufficient and the price is relatively expensive; it is relatively simple to grow seedlings with seeds and the cost is low; Or the cost of tissue culture is relatively high and the technique is difficult. Therefore, it is urgent to find a simple and fast tissue culture method of Panax notoginseng

[0004] Tissue culture is a technique that takes advantage of the totipotency of plant cells to cultivate seedlings from explants (such as stems, leaves, etc.) through the stages of callus induction, dedifferentiation and redifferentiation; Technical difficulties such as difficulty in inducing seedlings from injured tissue

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