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A strain of Clostridium panpan producing 4-methylphenol and its application

A technology of frying Clostridium and methylphenol, which is applied in the field of brewing and microbiology, and can solve the problems of low safety, low yield of 4-methylphenol, insufficient energy saving and environmental protection, etc.

Active Publication Date: 2022-03-18
BOZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The synthesis methods of 4-methylphenol mainly include chemical synthesis method and microbial fermentation method. The chemical synthesis method has the problems of low safety and insufficient energy saving and environmental protection, while the microbial fermentation method has the problem of low 4-methylphenol yield. A strain of Clostridium tyrobutyricum disclosed in CN106190924A, the output of its 4-methylphenol only reaches 0.13mg / L

Method used

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  • A strain of Clostridium panpan producing 4-methylphenol and its application
  • A strain of Clostridium panpan producing 4-methylphenol and its application
  • A strain of Clostridium panpan producing 4-methylphenol and its application

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Embodiment Construction

[0016] The screening and identification method of the producing bacteria of 4-methylphenol of the present invention are as follows:

[0017] 1 Materials and methods

[0018] 1.1 Materials and reagents

[0019] Choose different pit mud: No. 1 is the pit mud that does not produce the smell of pit mud (from the Wine Making Demonstration Training Center of Bozhou University), and No. 2 is the pit mud that produces the smell of pit mud (from the Wine Making Demonstration Training Center of Bozhou University) .

[0020] 1.2 Main instruments and equipment

[0021] WatersI-Class / XevoTQD gas chromatography-mass spectrometry (GC-MS); AgilentPC420 solid phase microextraction instrument; CAR / PDMS75μm headspace solid phase extraction head; BIOLOG microbial identification instrument, anaerobic workstation.

[0022] 1.3 Experimental method

[0023] 1.3.1 Separation and purification of microorganisms in pit mud with sugar as carbon source

[0024] 1.3.1.1 Preparation of culture medium

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Abstract

The invention discloses a strain of Clostridium sartagoforme bzxyb10007 producing 4-methylphenol and its application. The preservation name is Clostridium sartagoforme bzxyb10007, which has been preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2020623. The present invention determines the production bacteria of the defective flavor substance 4-methylphenol in Luzhou-flavor liquor, and provides theoretical and technical support for analyzing the formation mechanism of pit mud odor and constructing a strategy for eliminating mud odor, so that it can be cultivated from pit mud and The prevention and control of microorganisms that produce slimy odor should be started, and the elimination strategy of base wine slime odor should be constructed, so as to fundamentally solve the problem of the base wine of Luzhou-flavored liquor.

Description

technical field [0001] The invention relates to the technical fields of brewing and microbes, in particular to a strain producing 4-methylphenol, a defective flavor substance in Luzhou-flavor liquor. Background technique [0002] The liquor industry is a traditional industry with a history of thousands of years in my country. Its unique brewing process makes Chinese liquor have a unique flavor, rich in various aroma and taste compounds and functional active ingredients that are beneficial to health. Luzhou-flavor liquor is not only an integral part of my country's economic industry, but also an integral part of my country's long history and culture. With the rapid development of modern high-tech today, how to apply biotechnology to traditional crafts, on the basis of retaining the original cultural characteristics Realizing technological breakthroughs and innovations in the world is a concern of all wine industry workers. [0003] In recent years, with the rapid development ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12G3/02C12P7/22C12R1/145
CPCC12G3/02C12P7/22
Inventor 吴文睿方颂平刘露蒲顺昌刘开放董琪刘飞翔邢爽董书甲
Owner BOZHOU UNIV