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InDel marker fingerprint of shiitake Shenxiang 18 strain and construction method thereof

A fingerprint and construction method technology, applied in the field of detection of mushroom strains, can solve problems such as damage to the intellectual property rights of mushroom breeders, hidden dangers of mushroom industry development, and producer risks

Inactive Publication Date: 2021-04-23
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, the shiitake mushroom industry has become an important starting point for targeted poverty alleviation in China. According to statistics, more than 20% of national-level poverty-stricken counties in the country choose shiitake mushrooms as an important industry. The scale of shiitake mushroom cultivation in my country has further expanded. The problem of "same species with different names, different species with the same name" has become increasingly prominent, which not only damages the intellectual property rights of mushroom breeders, but also causes great risks for producers, and creates hidden dangers for the further development of mushroom industry

Method used

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  • InDel marker fingerprint of shiitake Shenxiang 18 strain and construction method thereof
  • InDel marker fingerprint of shiitake Shenxiang 18 strain and construction method thereof
  • InDel marker fingerprint of shiitake Shenxiang 18 strain and construction method thereof

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Experimental program
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Effect test

Embodiment 1

[0065] (1) Mycelia culture: transfer the mushroom mycelium to the potato glucose medium PDA, culture it in a darkened shake flask at 23-22°C, and collect the mycelium after 10 days;

[0066] (2) Extraction of genomic DNA: extract the genomic DNA of the above-mentioned hyphae with the CTAB method, detect the concentration and purity of the total genomic DNA by ultraviolet spectrophotometry, and adjust the concentration of the sample DNA to be 20 to 30 ng / uL;

[0067] The CTAB method for extracting the genomic DNA from mycelium includes:

[0068] ①Take the freeze-dried shiitake mycelia in a 2mL centrifuge tube, and put 2-3 steel balls into it;

[0069] ②Put the centrifuge tube into a multi-sample tissue grinder, set the frequency to 60Hz, and grind for 30s to a uniform powder;

[0070] ③ Add 2×CTAB extract preheated at 62°C for 1 hour, keep warm at 62°C for 60 minutes, and shake gently once every 20 minutes;

[0071] ④ Centrifuge at 12000rpm, 4°C for 20min, dispense the supern...

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Abstract

The invention relates to an InDel marker fingerprint of a shiitake Shenxiang 18 strain and a construction method thereof. The InDel marker fingerprint is composed of seven pairs of InDel markers developed after whole genome sequencing of the shiitake. The InDel marker fingerprint of the shiitake Shenxiang 18 strain can be used for specific identification of the shiitake Shenxiang 18 strain, has the advantages of short detection time, high accuracy and good repeatability, and has the specificity of the shiitake Shenxiang 18 strain among 44 collected shiitake strains.

Description

technical field [0001] The invention belongs to the detection field of mushroom strains, and in particular relates to an InDel marker fingerprint of mushroom Shenxiang No. 18 strain and a construction method thereof. Background technique [0002] Mushrooms (Lentinula edodes (Berk.) Pegler) are popular among consumers because of their high nutritional value and medicinal value. China is the first country in the world to carry out the artificial cultivation of shiitake mushrooms, with a cultivation history of more than 800 years. At present, shiitake mushrooms are the most widely cultivated and the highest yield mushroom species in China. According to statistics from the China Edible Fungi Association, the total output of edible fungi in China in 2018 was 38.4204 million tons, of which the total output of shiitake mushrooms was 10.4312 million tons, accounting for 27.12% of the total domestic output of edible fungi and more than 90% of the world's total output of shiitake mush...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12Q1/04C12N15/11C12R1/645
CPCC12Q1/6858C12Q1/6895C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 宋春艳于海龙章炉军尚晓冬谭琦张丹
Owner SHANGHAI ACAD OF AGRI SCI