Multi-peak coupling analysis method of cross response system based on DNA-AuNP coding

Abstract

The invention belongs to the field of biomedical sensing detection, and particularly relates to a multi-peak coupling analysis method of a cross response sensing system based on DNA-AuNP coding. The method comprises the following steps: modifying gold nanoparticles by polyA-DNA probes with different lengths to obtain a plurality of DNA-AuNPs with different binding capacities of polyA-DNA and the surfaces of the gold nanoparticles; completing the construction of the cross response sensor taking the aggregation degree of the polyA-DNAs modified gold nanoparticles with different lengths as a change factor; obtaining fingerprint information of different types of to-be-detected objects for inducing the aggregation degree of the gold nanoparticles by coupling the multi-peak signals; and classifying and identifying different types of the to-be-detected objects according to the fingerprint information. Aiming at the technical problems of tedious biochemical detection and classification process, strong specialty, high cost and easy interference of the existing metal ions, the invention provides the analysis method which is convenient to detect, time-saving, non-specific and high in sensitivity. Fingerprint information obtained by comparing body fluid of a normal person with body fluid of a patient can be detected, an aggregation degree database is constructed, and it is expected that the normal person and the patient are distinguished.

Description

technical field [0001] The invention belongs to the field of biomedical sensing and detection, in particular to a multi-peak coupling analysis method based on a DNA-AuNP coded cross-response system. Background technique [0002] Gold nanoparticle composites are widely used in the field of biomedical sensing and detection, but most of the existing characterization methods only consider the single mode characteristics of a single gold nanoparticle composite reaction caused by the test object, ignoring the relationship between different modal features. At the same time, in the process of data processing, the same weight is usually given to all data, and the information of weak correlation affects the quality of extracted features, resulting in the inability to achieve high-precision and high-sensitivity detection of detection objects. [0003] For example: the current common analysis method of gold nanoparticle complexes: ultraviolet spectrophotometry (UV), using the electronic...

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Problems solved by technology

[0004] In order to overcome the problem that the above-mentioned existing detection methods cannot realize high-precision and high-sensitivity detection, the present invention provides a multi-peak coupling analysis method based on a DNA-AuNP coded cross-response system, which can realize the detection of various analytes High-precision, high-sensitivity classification

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