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Preparation method of colloidal diamond carbon detection test strip for Sudan red I in food

A technology for detecting test strips and Sudan red, used in biological testing, measuring devices, material inspection products, etc.

Inactive Publication Date: 2021-04-23
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems existing in the Sudan I colloidal gold detection test strips at the present stage, the invention provides a preparation method for the Sudan I colloidal diamond carbon detection test strips in food, which solves the problem of the T line on the traditional test strips. The problem of overlapping red and colloidal gold red has certain reference value for the production of other test strips, and it can be clearly judged, quickly and accurately judged, and the operation is simple

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of preparation method of Sudan red I colloidal diamond carbon detection test strip in food is characterized in that comprising the following steps:

[0031] S1: Preparation of colloidal carbon solution: dissolve glucose in dilute alkali solution, add amide sugar at a temperature of 170°C and a pressure of 1 MPa, react for 10 minutes, centrifuge, wash, and freeze-dry to obtain black colloidal carbon powder, and colloidal carbon powder Ultrasonic at the end, activated with epoxy molecules under alkaline conditions, and configured into a solution;

[0032] Colloidal carbon gold tempering: Carry out colloidal carbon solution for adamantization, boil the colloidal carbon solution under a pressure of 3 atmospheres for 30 minutes, then ultrasonicate at 40KHZ for 30 minutes, disperse, and finally dilute it into a standard concentration of colloidal adamantine carbon solution;

[0033] S2: Take 100mL of colloidal gold steel carbon solution with a particle size of 40nm, ad...

Embodiment 2

[0039] S1: Preparation of colloidal carbon solution: dissolve sucrose in dilute alkali solution, add amide sugar at a temperature of 190°C and a pressure of 1.2 MPa, react for 70 minutes, centrifuge, wash, and freeze-dry to obtain black colloidal carbon powder, and colloidal carbon The powder is ultrasonicated, activated with epoxy molecules under alkaline conditions, and configured into a solution;

[0040] Colloidal carbon gold tempering: Carry out colloidal carbon solution for adamantization, boil the colloidal carbon solution for 35 minutes under a pressure of 3 atmospheres, then ultrasonicate for 35 minutes at 50KHZ, disperse, and finally dilute it into a standard concentration of colloidal adamantine carbon solution;

[0041]S2 is identical with embodiment 1;

[0042] S3: First centrifuge the carbon-labeled antibody solution at room temperature and 1300rpm for 25min, take the black supernatant solution, and then centrifuge at 4°C for 30min at 8000r / min. After centrifugat...

Embodiment 3

[0045] S1: Preparation of colloidal carbon solution: dissolve fructose in dilute alkali solution, add amide sugar at a temperature of 210°C and a pressure of 1.5 MPa, react for 120 minutes, centrifuge, wash, and freeze-dry to obtain black colloidal carbon powder, and colloidal carbon The powder is ultrasonicated, activated with epoxy molecules under alkaline conditions, and configured into a solution;

[0046] Colloidal carbon gold tempering: Carry out colloidal carbon solution for adamantization, boil the colloidal carbon solution under a pressure of 3 atmospheres for 40 minutes, then ultrasonicate at 60KHZ for 40 minutes, disperse, and finally dilute it into a standard concentration of colloidal adamantine carbon solution;

[0047] S2 is identical with embodiment 1;

[0048] S3: First, centrifuge the carbon-labeled antibody solution at room temperature and 1500rpm for 30min, take the black supernatant solution, and then centrifuge at 4°C for 30min at 8000r / min. After centrif...

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Abstract

The invention belongs to the technical field of food safety detection, and particularly relates to a preparation method of a colloidal diamond carbon detection test strip for Sudan red I in a food. According to the method, colloidal diamond carbon serving as a black marker is used for replacing colloidal gold for color development, obvious advantages are achieved, a detection line is reddish and positive, and a detection line is black and negative. Therefore, the advantages of rapidness, simplicity in operation and the like of an immune method are kept, and the sensitivity and the specificity are improved.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a preparation method of a Sudan red I colloidal diamond carbon detection test strip in food. Background technique [0002] Sudan red substances contain nitrogen-nitrogen double bonds (azo groups), that is, -N=N- in the structural formula, and together with the benzene rings connected to its two ends constitute a chromophoric group. The large conjugated system of azobenzene composed of azo groups and aromatic structures can absorb visible light of a certain wavelength, so substances containing azobenzene systems can show certain colors. Sudan Red I (structural formula is as follows), scientific name: 1-phenylazo-2-naphthalenol (1-phenylazo-2-naphthalenol), molecular structural formula is C6H5N=NC10H6OH, molecular weight 248.28. [0003] Sudan red is a lipophilic azo dye, insoluble in water, mainly used for dyeing oil paint, engine oil, wax and shoe polis...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/577G01N33/558G01N33/532G01N33/531G01N33/53
Inventor 殷奎德张兴梅韩琰旭张科陈发荣吴月皓齐瑜
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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