A functional material for degrading and measuring tetracycline, its preparation method and application
A functional material, tetracycline technology, applied in chemical instruments and methods, through the chemical reaction of materials to analyze, analyze materials, etc., can solve complex equipment, time-consuming and other problems, achieve high signal-to-noise ratio, simple preparation method, Effect of Improving Measurement Sensitivity
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Embodiment 1
[0030] Example 1 Preparation of functional material AgNP-HPA-Tb for degradation and determination of tetracycline
[0031] Preparation of silver nanoparticles: To 4 mL of ethylene glycol, add 1 mL of 0.1 M AgNO 3 Aqueous solution and 4 mL aqueous solution containing 0.04 g polyvinylpyrrolidone were stirred for 20 minutes, the solution was transferred to a stainless steel reaction kettle lined with tetrafluoroethylene, heated at 180°C for 5 hours, cooled to room temperature, and centrifuged to separate the precipitate. Washed 6 times with water and ethanol, and dried at 80°C for 1 hour to obtain figure 1The silver nanoparticles shown in A have a particle size of about 50-200 nm.
[0032] Preparation of AgNP-HPA-Tb: To 5 mL N, N-dimethylformamide (DMF), add 1 mL 30 mM 3-hydroxy-2-pyridinecarboxylic acid in DMF solution, 1 mL 20 mM Tb(NO 3 ) 3 Aqueous solution, after stirring and mixing, add 2 mL of ethanol solution containing 0.01 g of silver nanoparticles prepared above, and...
Embodiment 2
[0033] Example 2 Preparation of functional material AgNP-HPA-Tb material for degradation and determination of tetracycline
[0034] To 5 mL of N,N-dimethylformamide (DMF), sequentially add 1 mL of 30 mM 3-hydroxy-2-pyridinecarboxylic acid in DMF, 1 mL of 20 mM Tb(NO 3 ) 3 Aqueous solution, after stirring and mixing, add 2 mL of ethanol solution containing 0.05 g of silver nanoparticles prepared in Example 1, magnetically stir evenly, terbium ion in the mixed solution: 3-hydroxyl-2-pyridinecarboxylic acid: the molar ratio of silver nanoparticles is 1:1:5, stirred for 10 minutes, the mixture was transferred to a stainless steel reaction kettle, reacted at 140°C for 5 hours, cooled to room temperature, centrifuged to separate the precipitate, washed 3 times with ethanol and pure water, and placed in 60 ℃ drying oven for later use. figure 1 C is the scanning electron microscope image of the prepared AgNP-HP-Tb, and the AgNP-HPA-Tb material is in the form of network-wrapped nanop...
Embodiment 3
[0035] Example 3 Application of functional material AgNP-HPA-Tb to degrade tetracycline
[0036] Take 10 μL of 0.01 M tetracycline hydrochloride aqueous solution and 10 μL of the AgNP-HPA-Tb suspension (0.3 mg / mL) prepared in Example 2, and add it to 970 μL of HEPES (N-2-hydroxyethylpiperazine-N'-2 -ethanesulfonic acid) buffer solution (10 mM, pH7), then add 10 μL of 10 mM hydrogen peroxide solution, mix well and react for 20 minutes, measure the ultraviolet-visible absorption spectrum of the tetracycline solution every few minutes. figure 2 It is the ultraviolet-visible absorption spectrum of tetracycline under the action of AgNP-HPA-Tb. The absorption peaks of tetracycline at 275 nm and 358 nm disappear, indicating that tetracycline is completely degraded. image 3 is the degradation curve of tetracycline, and the absorbance of tetracycline continuously reduces along with the carrying out of reaction, under the situation of not using hydrogen peroxide, after 120 minutes, th...
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