Mutants of paenibacillus and methods for their use

A technology of Bacillus and Paenibacillus, applied in the field of bacterial strains and their ability to control plant diseases, can solve problems such as expensive and cumbersome procedures

Pending Publication Date: 2021-04-30
BAYER CROPSCI LP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Costly and burdensome procedures may be required to remove EPS from large-scale broth cultures prior to further processing

Method used

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  • Mutants of paenibacillus and methods for their use
  • Mutants of paenibacillus and methods for their use
  • Mutants of paenibacillus and methods for their use

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[0069] In one embodiment, mutant strains of Paenibacillus sp. strain NRRL B-67304, Paenibacillus sp. strain NRRL B-67306, or Paenibacillus sp. strain NRRL B-67615 are provided. The term "mutant" refers to a genetic variant derived from Paenibacillus sp. strain NRRL B-67304, Paenibacillus sp. strain NRRL B-67306, or Paenibacillus sp. strain NRRL B-67615. In one embodiment, the mutant has one or more of Paenibacillus sp. strain NRRL B-67304, Paenibacillus sp. strain NRRL B-67306, or Paenibacillus sp. strain NRRL B-67615 or All identifying (functional) characteristics. In a specific example, the mutant or its fermentation product controls at least as much as the parent Paenibacillus sp. strain NRRL B-67304, Paenibacillus sp. strain NRRL B-67306 or Paenibacillus sp. strain NRRL B-67615. (as identified functional characteristics) fungi, oomycetes and / or bacteria. Such a mutant may be a genetic variant having a genomic sequence that shares the same genomic sequence as Paenibacillu...

Embodiment 1

[0143] Example 1. Enhancing Fusaridin Production by Paenibacillus sp. Strain NRRL B-67129 and Mutant Derivatives thereof

[0144] Paenibacillus sp. strain NRRL B-67129 was treated with 1-methyl-3-nitro-1-nitroguanidine (NTG) or ethyl methanesulfonate (EMS) to introduce genetic variation. Treatments that resulted in a 50-90% loss of colony forming units (CFU) were considered suitable for obtaining sufficient genetic variation and viable cells for subsequent screening. Individual isolates from chemically treated populations were grown in 96-well deep-well plates (blocks) and screened for increased production of four fusicidin-like compounds, fusaricidin A (also known as " Fus A"), LiF08a, Paeniserine A1 and B1 (also called "M868" for their molecular weight); and Paeniprolixin A2 and B2 (also called "M938" for their molecular weight) as described in WO 2016 / 154297 ).

[0145] Isolates from this initial screen with significantly increased levels of fusaricidin were rescreened in...

Embodiment 2

[0152] Example 2 Reduction of the fermentation broth viscosity of Paenibacillus strain NRRL B-67129 mutant derivatives

[0153] Paenibacillus sp. strain NRRL B-50972 and Paenibacillus sp. strain NRRL B-67129 produced viscous broth cultures, as did many of the mutant strains derived from Paenibacillus sp. strain NRRL B-67129. The physicochemical properties of these cultures present challenges for fermentation and downstream processing. Therefore, there is a need to identify a method for identifying mutant derivatives of Paenibacillus sp. strain NRRL B-67129 that produce less viscous broth cultures.

[0154] Sucrose is frequently used by Paenibacillus species as a carbon source for exopolysaccharide (EPS) production, and the use of sucrose has been reported to result in a significant yield of high molecular weight Levan-type EPS (Liang and Wang. Mar. Drugs 2015, 13, 1847-1863). High molecular weight EPS polymers have found commercial use as thickeners. Along with sucrose, oth...

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Abstract

The present invention provides a composition comprising a biologically pure culture of a Paenibacillus sp. strain comprising a mutant DegU lacking a functional receiver domain or a functional DNA binding domain and / or a mutant DegS lacking a functional single binding domain or a functional ATPase domain with decreased viscosity in a liquid culture. Also provided is a method of identifying a Paenibacillus sp. mutant derivative strain with decreased viscosity in a liquid culture compared to a Paenibacillus sp. parental strain with a visual screen for mutant isolates with a non-mucoid morphology.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application No. 62 / 671,067, filed May 14, 2018, the contents of which are incorporated herein by reference in their entirety. [0003] References to Sequence Listings Submitted Electronically [0004] An official copy of the Sequence Listing is submitted electronically via EFS-Web as a Sequence Listing in ASCII format with the file name "BCS169009_WO_ST25.txt", created on April 30, 2019, 29 kilobytes in size, and the The official copy is submitted together with the specification. The Sequence Listing contained in this file in ASCII format is part of the specification and is hereby incorporated by reference in its entirety. technical field [0005] The present invention relates to the field of bacterial strains and their ability to control plant diseases. In particular, the present invention relates to Paenibacillus sp. strains having relatively high levels of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C07K14/195C12Q1/04A01N63/25A01P3/00C12R1/01
CPCC07K14/195C12N1/20A01N63/25C12R2001/01C12N1/205
Inventor J·A·科林斯J·F·桑切斯J·L·沃勒B·A·塔格S·K·霍顿T·J·泰格勒
Owner BAYER CROPSCI LP
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