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Beta-carotene embedding emulsion based on soybean peptide aggregates and preparation method thereof

A technology of carotene and soybean peptide, applied in the direction of food ingredients as emulsifiers, food science, application, etc., can solve the problems of consumer inconvenience, reduced applicability, and vulnerability to losses, and achieve the improvement of antioxidant capacity, emulsifying activity and Improvement of emulsification stability and improvement of nutritional value

Inactive Publication Date: 2021-05-11
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

β-carotene is so important, but it is sensitive to external conditions such as light, heat, oxygen, etc. If it is not properly protected during storage, it will easily suffer losses, greatly reducing its applicability, and bringing a lot of inconvenience to consumers

Method used

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  • Beta-carotene embedding emulsion based on soybean peptide aggregates and preparation method thereof
  • Beta-carotene embedding emulsion based on soybean peptide aggregates and preparation method thereof
  • Beta-carotene embedding emulsion based on soybean peptide aggregates and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] (1) Soybean protein isolate solution was stirred at a solid-liquid ratio of 1:20g / mL for 2 hours, then hydrated overnight, and heated at 90°C for 15 minutes to adapt to denaturation and facilitate enzymatic hydrolysis. Then it was hydrolyzed at 55°C and pH 8.0 for 6h. After enzymatic hydrolysis, treat at 100°C for 5 minutes, immediately adjust the pH to 7.0 in an ice bath to room temperature, and centrifuge at 4000 rpm / min for 15 minutes to obtain soybean peptide aggregates (SPA), wash twice with water, redisperse, and freeze-dry. The SPA solution was magnetically stirred at room temperature for 2 h, then placed at 4°C overnight to fully hydrate, and the pH was adjusted to 7.0 with 0.1 mol / L NaOH or HCl. Ultrasonic treatment was performed under the conditions of ultrasonic power 150W and output frequency 20kHz. Centrifuge at 8000rpm / min for 10min after the ultrasonication to remove a small amount of insoluble precipitate, and the obtained particle solution is soybean p...

Embodiment 2

[0035] (1) Soybean protein isolate solution was stirred at a solid-liquid ratio of 1:20g / mL for 2 hours, then hydrated overnight, and heated at 90°C for 15 minutes to adapt to denaturation and facilitate enzymatic hydrolysis. Then it was hydrolyzed at 55°C and pH 8.0 for 6h. After enzymatic hydrolysis, treat at 100°C for 5 minutes, immediately adjust the pH to 7.0 in an ice bath to room temperature, and centrifuge at 4000 rpm / min for 15 minutes to obtain soybean peptide aggregates (SPA), wash twice with water, redisperse, and freeze-dry. The SPA solution was magnetically stirred at room temperature for 2 h, then placed at 4°C overnight to fully hydrate, and the pH was adjusted to 7.0 with 0.1 mol / L NaOH or HCl. Ultrasonic treatment was performed under the conditions of ultrasonic power 150W and output frequency 20kHz. Centrifuge at 8000rpm / min for 10min after the ultrasonication to remove a small amount of insoluble precipitate, and the obtained particle solution is soybean p...

Embodiment 3

[0042] (1) Soybean protein isolate solution was stirred at a solid-liquid ratio of 1:20g / mL for 2 hours, then hydrated overnight, and heated at 90°C for 15 minutes to adapt to denaturation and facilitate enzymatic hydrolysis. Then it was hydrolyzed at 55°C and pH 8.0 for 6h. After enzymatic hydrolysis, treat at 100°C for 5 minutes, immediately adjust the pH to 7.0 in an ice bath to room temperature, and centrifuge at 4000 rpm / min for 15 minutes to obtain soybean peptide aggregates (SPA), wash twice with water, redisperse, and freeze-dry. The SPA solution was magnetically stirred at room temperature for 2 h, then placed at 4°C overnight to fully hydrate, and the pH was adjusted to 7.0 with 0.1 mol / L NaOH or HCl. Ultrasonic treatment was performed under the conditions of ultrasonic power 150W and output frequency 20kHz. Centrifuge at 8000rpm / min for 10min after the ultrasonication to remove a small amount of insoluble precipitate, and the obtained particle solution is soybean p...

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Abstract

The invention discloses a preparation method of a beta-carotene embedding emulsion based on soybean peptide aggregates, which comprises the following steps: preparing a soybean peptide nanoparticle solution: preparing soybean peptide aggregates from a soybean protein isolate solution by an enzyme hydrolysis method, and performing ultrasonic treatment to obtain the soybean peptide nanoparticle solution for later use; preparing soybean peptides with different hydrolysis degrees: using protease to hydrolyze the soybean protein isolate solution, and centrifuging to remove part of insoluble impurities for later use; preparing an oil phase: dissolving beta-carotene in soybean oil to prepare the oil phase for later use; preparing a water phase: uniformly mixing the prepared soybean peptide nanoparticle solution and the prepared soybean peptide solution to prepare the water phase for later use; mixing: mixing the prepared oil phase and the prepared water phase, and dispersing at a high speed; and homogenizing: homogenizing the obtained product to obtain the product emulsion. The composite emulsion is prepared according to the advantages of the soybean peptide aggregates and the soybean peptides, beta-carotene can be well protected, and loss is reduced as much as possible.

Description

technical field [0001] The invention relates to a beta-carotene embedding emulsion based on soybean peptide aggregates and a preparation method thereof. Background technique [0002] The color of β-carotene is very attractive. Due to the difference in concentration, its color can show all colors from red to yellow, and its coloring ability is strong, so it can be used as a food additive. It is found in fruits and vegetables such as mangoes, tomatoes, carrots, and spinach. β-carotene is an excellent food additive, but our body cannot synthesize it by itself and must be obtained from other fruits and vegetables. [0003] Studies have shown that β-carotene can also improve the body's defense against diseases, and appropriate supplementation of β-carotene can reduce the incidence of heart disease and cancer. The lack of β-carotene in the human body will cause the corneal epithelium to fall off, the keratinization will deepen, and at the same time, the growth and differentiatio...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23L33/18A23L29/10A23J3/34
CPCA23L33/105A23L33/18A23L29/10A23J3/346A23V2002/00A23V2250/211A23V2250/55A23V2200/222
Inventor 丁秀臻王丹孟娇代养勇赵路苹李向阳
Owner SHANDONG AGRICULTURAL UNIVERSITY
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