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Stem cell culture method

A technology of stem cell culture and culture medium, applied in the field of stem cell culture

Inactive Publication Date: 2021-05-11
王墨权
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Including console II, culture medium tank, abutment, base column, magnetic part, cover seat, hinge part, cover and clamping part, the mixing force can be fine-tuned and routinely adjusted during the preparation of culture medium, and the soft clamp and The combined use of the card joint can change the operation of mixing and centrifugation by changing different usage methods; the disadvantage of this invention is that it cannot perform centrifugal separation at different levels

Method used

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Examples

Experimental program
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specific Embodiment approach 1

[0035] Combine below Figures 1 to 9 Describe this embodiment mode, a kind of stem cell culture method, this method comprises the following steps:

[0036] Step 1: placing a plurality of culture test tubes 408 filled with culture fluid on the centrifugal mechanism 4;

[0037] Step 2: the rotating mechanism 2 drives a plurality of centrifugal mechanisms 4 to rotate, and centrifugally stratifies the culture solution;

[0038] Step 3: multiple extracting mechanisms 5 respectively peel off the layered culture solutions in multiple culture test tubes 408;

[0039] The above-mentioned stem cell culture method also relates to a stem cell culture device. The stem cell culture device includes a device bracket 1, a rotating mechanism 2, a pushing mechanism 3, a centrifugal mechanism 4, an extracting mechanism 5 and an angle motor 6. The device bracket 1 is connected with a rotating mechanism 2. The pushing mechanism 3 is fixedly connected to the device bracket 1, and the pushing mecha...

specific Embodiment approach 2

[0041] Combine below Figures 1 to 9Describe this embodiment, this embodiment will further explain Embodiment 1, the device bracket 1 includes a bottom bracket 101, a support ring I 102, an installation bracket 103 and a support ring II 104, the middle part of the bottom bracket 101 is fixedly connected with a support ring I 102, the bottom A mounting bracket 103 is fixedly connected to the bottom of the bracket 101 , and a supporting ring II 104 is fixedly connected to the upper end of the mounting bracket 103 .

specific Embodiment approach 3

[0043] Combine below Figures 1 to 9 Describe this embodiment, this embodiment will further explain the second embodiment, the rotating mechanism 2 includes a rotating motor 201, a rotating cylinder I202, a rotating pulley I203, a slide rail I204, a transmission shaft 205, a rotating cylinder II206, a rotating pulley II207 and slide rail II208, the rotating motor 201 is fixedly connected to the bottom bracket 101, the rotating cylinder I202 is rotationally connected to the support ring I102, the rotating cylinder I202 is fixedly connected to the rotating pulley I203, the output of the rotating pulley I203 and the rotating motor 201 Shaft transmission connection, four sliding rails I204 are fixedly connected to the rotating cylinder I202, the transmission shaft 205 is rotationally connected to the bottom bracket 101, the transmission shaft 205 is connected to the rotating pulley I203, and the rotating cylinder II206 is rotationally connected to the supporting ring II104. The ro...

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Abstract

The invention relates to stem cell culture, in particular to a stem cell culture method. The stem cell culture method comprises the following steps of: S1: placing a plurality of culture test tubes filled with culture liquid on centrifugal mechanisms; S2: driving a plurality of centrifugal mechanisms by a rotary mechanism to rotate to carry out centrifugal layering on the culture liquid; and S3: independently stripping the layered culture liquid in the plurality of culture test tubes by a plurality of extraction mechanisms. The plurality of culture test tubes filled with the culture liquid are placed on the centrifugal mechanisms, the rotary mechanism drives the plurality of centrifugal mechanisms to rotate to carry out the centrifugal layering on the culture liquid, a pushing mechanism pushes the plurality of centrifugal mechanisms to move so as to regulate the centrifugal radii of the plurality of centrifugal mechanisms, an angle motor drives the centrifugal mechanisms to rotate so as to regulate the centrifugal angle of each centrifugal mechanism, and the plurality of extraction mechanisms independently strip the layered culture liquid in the plurality of culture test tubes.

Description

technical field [0001] The present invention relates to stem cell culture, more specifically a stem cell culture method. Background technique [0002] For example, the publication number CN111172014A is a stem cell culture fluid preparation device and a stem cell culture fluid preparation method. Including console II, culture medium tank, abutment, base column, magnetic part, cover seat, hinge part, cover and clamping part, the mixing force can be fine-tuned and routinely adjusted during the preparation of culture medium, and the soft clamp and The combined use of the clamping part can change the operation of mixing and centrifugation by changing different usage modes; the disadvantage of this invention is that it cannot carry out centrifugation of different levels. Contents of the invention [0003] The purpose of the present invention is to provide a method for culturing stem cells, which can rapidly perform centrifugation at different levels. [0004] The purpose of t...

Claims

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Application Information

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IPC IPC(8): C12M1/24C12M1/00B04B5/04B04B7/08B04B9/04B04B11/04
CPCB04B5/0414B04B7/08B04B9/04B04B11/04C12M23/08C12M99/00
Inventor 王墨权
Owner 王墨权
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