Marker for pre-judging severe new coronal pneumonia (COVID-19) and product and application thereof

A marker, critically ill technology, applied in recombinant DNA technology, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of many detection factors, complex prediction process, and unsatisfactory single prediction effect. To achieve the effect of reducing the fatality rate and simplifying the detection process

Pending Publication Date: 2021-05-14
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the single prediction effect of these factors is not ideal, and it is often necessary to consider multiple factors comprehensively, which makes the prediction process very complicated
There are also some researchers who study the proteome, transcriptome, metabolome, etc. through omics technology, and form a single-omics or multi-omics biomarker set on this basis, but because there are too many detection factors, it is not practical in clinical practice. Low reliability and few experimental replications

Method used

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  • Marker for pre-judging severe new coronal pneumonia (COVID-19) and product and application thereof
  • Marker for pre-judging severe new coronal pneumonia (COVID-19) and product and application thereof
  • Marker for pre-judging severe new coronal pneumonia (COVID-19) and product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1S100A12 as a severe infection marker

[0050]Collect 13 sets of relevant peripheral blood transcriptome data from the public platform, and conduct in-depth analysis on the gene expression data of 1648 samples. These data include data related to sepsis, data related to influenza, and data related to new coronary pneumonia.

[0051] (1) S100A12 as a marker of severe infection

[0052] From figure 1 It can be seen that the expression level of S100A12 (SEQ ID NO.1) is significantly increased in general infection (compared with the healthy group) (p=1.95e-8). In the sepsis group, the expression level of S100A12 further increased (p=0.0074). Therefore, S100A12 can be used as a marker of severe infection.

[0053] (2) Correlation between the expression level of S100A12 and the severity of sepsis

[0054] From figure 2 It can be seen that the expression level of S100A12 in the mild sepsis group was significantly higher than that in the SIRS group (ie patients w...

Embodiment 2

[0076] Example 2 A method of predicting severe new coronary pneumonia

[0077] (1) The primer synthesis of the target gene S100A12 and the reference gene B2M was entrusted to Shanghai Sangong. The specific sequence is as follows:

[0078] S100A12-F SEQ ID NO.2 S100A12-R SEQ ID NO.3 B2M-F SEQ ID NO.4 B2M-R SEQ ID NO.5

[0079] (2) Collect 100 μL of venous blood from the subject (50 μL or fingertip blood can also be collected here).

[0080] (3) Mix whole blood with three volumes of lysate (Bitec whole blood lysate). The RNA was purified with a kit (Bitek Whole Blood RNA Purification Kit) and the RNA concentration and quality were detected.

[0081] (4) Reverse transcription kit of Takara, product number RR037A was used for reverse transcription; kit of Thermo Fisher Corporation was used for PCR amplification, product number was A44647100. In this way, the Ct values ​​of the target gene and the internal reference gene were obtained.

[00...

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Abstract

The invention provides a marker for pre-judging a severe disease. The marker for pre-judging the severe disease is S100A12, the sequence of the marker is SEQ ID NO.1, and the severe disease is one of severe new coronal pneumonia and severe infection. The S100A12 gene is used as a marker, the expression level of the S100A12 gene in whole blood is detected when severe diseases are pre-judged, leukocytes do not need to be separated, and the detection process is simplified. The expression level of the S100A12 can guide the pre-judgment of infectious diseases including severe new coronal pneumonia, so that the infectious diseases can be treated as soon as possible, the case fatality rate is reduced, and the S100A12 has a good clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a marker for predicting severe new coronary pneumonia (COVID-19) and its product and application. Background technique [0002] Novel coronavirus pneumonia (COVID-19) is a pneumonia caused by a new type of coronavirus SARS-COV-2. Most infected people have mild or no symptoms, and a small number of infected people can develop severe or critical illness. Predicting the development of the infected person's condition as early as possible can provide timely intervention for patients who are likely to develop severe and critical illness, and reduce the mortality rate of new coronary pneumonia. [0003] Detect some commonly used immune indicators, and conduct correlation analysis with the condition and trend of new coronary pneumonia, and find that some immune indicators can help predict severe new coronary pneumonia, including CRP, PCT, d-dimer, ferritin, LDH, fibri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/166Y02A50/30
Inventor 雷红星
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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