Novel coronavirus detection kit as well as detection method and application thereof

Abstract

The invention belongs to the field of biological medicines, and particularly relates to a novel coronavirus neutralizing antibody detection kit prepared by adopting a Pt-Pd alloy nano-particle labeled immunochromatography method, and a detection method and application of the novel coronavirus neutralizing antibody detection kit. The kit comprises a novel coronavirus SARS-CoV-2 neutralizing antibody Pt-Pd alloy nanoparticle test strip. The preparation method of the novel coronavirus SARS-CoV-2 neutralizing antibody Pt-Pd alloy nanoparticle test strip comprises the following steps: marking a novel coronavirus SARS-CoV-2 antigen as a capture antibody nano marker by using alloy nanoparticles, coating a purified A-type expression antigen and an antibody thereof on a cellulose membrane to respectively serve as a detection line T line and a quality control line C line, and performing condition optimization to establish the novel coronavirus neutralizing antibody Pt-Pd alloy nanoparticle test strip. The kit is convenient to operate and carry, has relatively high sensitivity, has operability and reproducibility, and can be used for diagnosing pneumonia caused by novel coronavirus, and the sensitivity of the kit can be improved by 24 times compared with that of colloidal gold.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to an antibody detection kit, in particular to a novel coronavirus neutralizing antibody detection kit prepared by immunochromatography labeled with Pt-Pd alloy nanoparticles and its detection method and application. Background technique [0002] Since the outbreak of novel coronavirus pneumonia (Corona Virus Disease 2019, COVID-19), it has rapidly swept across most countries around the world and continues to spread. As of February 27, 2021, the number of confirmed cases in China is 523, and the cumulative number of confirmed cases is 101,872; the number of confirmed cases abroad is 24,682,961, and the cumulative number of confirmed cases is 113,938,891. Whether it is the detection, prevention or treatment of the new crown, countries are under tremendous pressure. [0003] The diagnostic kits that have been developed so far can be divided into two categories according to the detection targe...

AI Technical Summary

Problems solved by technology

However, these diagnostic kits still have many limitations, and the false negative rate is too high, so that they cannot fully meet the needs of epidemic prevention and clinical work

Especially the needs of epidemiological investigation and general inspection: the specificity of nucleic acid detection kits is close to 100%, but due to sampling difficulties and other reasons, the sensitivity is low, only 30%-50%, that is, there are many missed detections: use The sensitivity of antibody detection kits for SARS-CoV-2 constituent proteins such as S protein and N protein can reach more than 95%, but the specificity is low due to principle defects (especially for patients with some diseases, non-SARS- CoV-2 antibodies can also be recognized by these constituent proteins), that is, there are more false detections

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