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Particle measuring device, calibration method, and measuring device

A technology of measuring device and calibration method, applied in measuring device, particle size analysis, individual particle analysis, etc., can solve problems such as difficulty in temperature measurement

Pending Publication Date: 2021-05-25
NAT INST OF ADVANCED IND SCI & TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it is difficult to measure the temperature in places observed with a microscope

Method used

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  • Particle measuring device, calibration method, and measuring device
  • Particle measuring device, calibration method, and measuring device
  • Particle measuring device, calibration method, and measuring device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0027] figure 1 It is a diagram showing the configuration of the particle measuring device according to Embodiment 1 of the present invention. The particle measuring device of Embodiment 1 measures the particle diameter of target particles in a dispersion medium. like figure 1 As shown, the particle measurement device according to Embodiment 1 includes a measurement system 1 and a calculation processing device 2 . The measurement system 1 includes an optical unit 11 and an imaging device 12 . The optical unit 11 is an optically transparent unit in which a dispersion medium is retained or circulated. The optical unit 11 is, for example, a flow cell. The imaging device 12 performs optical imaging with an imaging element (CCD: Charge Coupled Device, CMOS image sensor, etc.) through an optical system such as a lens, and outputs image data of a captured image of pixels arranged two-dimensionally at a predetermined resolution. The imaging device 12 captures each captured image ...

Embodiment approach 2

[0066] According to Embodiment 1, although the division correction between the length of the pixel unit in the captured image and the length of the physical unit (meter unit in the SI unit system, etc.) is not essential, the same correction particles can be used Perform calibration. In Embodiment 2, by using a previously obtained dispersion medium with a viscosity η and a predetermined particle diameter d cal Graduation calibration of the measurement device (optical microscope, etc.) that takes an image of the object and measures it with an imaging device.

[0067] Figure 5 It is a figure which shows an example of the measurement apparatus calibrated by the calibration method of Embodiment 2. Figure 5 The shown measurement device includes a measurement system 61 and a calculation processing device 62 . The measurement system 61 includes an imaging device 71 . The imaging device 71 is the same as the imaging device 12 described above.

[0068] The calculation processing ...

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Abstract

The objective of the present invention is to enable calibration of a measuring device which captures an image of a target object such as a particle of interest to be performed simply, using a calibration particle. An image analyzing unit (21) acquires a plurality of captured images obtained with a time interval deltat, and (a), in a calibration mode, determines a mean squared displacement deltaMS-cal of a pixel-unit bright spot in a calibration particle, on the basis of the displacement of the bright spot in the calibration particle in the plurality of captured images, and (b), in a measuring mode, determines a mean squared displacement deltaMS of a bright spot in particle of interest, on the basis of the pixel-unit displacement of the bright spot in the particle of interest in the plurality of captured images. Furthermore, (c), in an analysis mode, a particle diameter analyzing unit (22) derives a particle diameter d of the particle of interest from the mean squared displacement deltaMS of the bright spot in the particle of interest, on the basis of the mean squared displacement deltaMS-cal of the bright spot in the calibration particle, and a particle diameter dcal of the calibration particle.

Description

technical field [0001] The present invention relates to a particle measuring device, a calibration method, and a measuring device. Background technique [0002] As a method of measuring the particle diameter of the particles in the dispersion medium, there is the PTA (Particle Tracking Analysis) method, which is to irradiate the particles with laser light, and use an optical system to observe the condensing point (light spot) of the scattered light of the particles irradiated by the laser light, The particle size is calculated from the Brownian motion of each particle using the Stokes-Einstein formula. [0003] In the PTA method, the center of gravity of the light spot is calculated for a series of images taken by a camera at a time interval Δt, and the trajectory of the Brownian motion of the particle is obtained by corresponding the light spots of adjacent frames, and according to the relational expression (Δ MS =4DΔt), from the above 2-dimensional mean square displacemen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/02G01N15/00
CPCG01N15/1012G01N15/0227G01N15/1459G01N2015/1493G01N15/1429G01N2015/0053G01N2015/1486G01N15/06G01N2015/0687G01N15/1433G01N15/075G01N15/14G06T5/00G06T7/80G06T7/20G06T7/60G06T2207/10016
Inventor 加藤晴久松浦有祐中村文子近藤郁田渕拓哉冨田宽林秀和
Owner NAT INST OF ADVANCED IND SCI & TECH