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Method for enriching and purificying four alkaloid components in uncaria rhynchophylla

A purification method and alkaloid technology, which is applied in the field of alkaloids, can solve the problems of no extraction and purification methods, strong toxicity and side effects, and low content of alkaloid compounds, and achieve the effects of easy enrichment, clear activity, and low production cost

Pending Publication Date: 2021-05-28
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The content of alkaloid compounds in Uncaria uncariae is low and there is no mature extraction and purification method. At present, it is mainly obtained by chemical synthesis. There are strong toxic and side effects when chemical compounds are synthesized. Therefore, it is established to extract and purify this compound from natural plants. The compound-like method has great practical significance

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  • Method for enriching and purificying four alkaloid components in uncaria rhynchophylla

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Uncaria stems are dried and crushed, weighed 100g, soaked in 1000mL volume concentration 70% ethanol for 12h, ultrasonicated for 30min, filtered, and the filtrate was stored for later use; repeat the above extraction process 5-6 times, combined the filtrate, concentrated under reduced pressure to remove ethanol to obtain the crude extract of Uncaria. Extract with the same volume of petroleum ether as the crude extract, extract 4 times in total, remove the fat-soluble components, extract with ethyl acetate with the same volume as the crude extract, extract 4 times in total, and concentrate the ethyl acetate extract under reduced pressure to obtain Extract, after dissolving the extract with 20% volume concentration ethanol, use D3520 macroporous adsorption resin to pack and separate, and then use 6 times column volume of water, volume concentration 10%, 20%, 30%, 40%, 50% %, 60%, 70%, 80%, 90%, and 100% ethanol were eluted, and the eluted parts with a volume concentration...

Embodiment 2

[0041] Uncaria stems are dried and crushed, weighed 100g, soaked in 1000mL volume concentration 70% ethanol for 12h, ultrasonicated for 30min, filtered, and the filtrate was stored for later use; repeat the above extraction process 5-6 times, combined the filtrate, concentrated under reduced pressure to remove ethanol to obtain the crude extract of Uncaria. Extract with the same volume of petroleum ether as the crude extract, extract 4 times in total, remove the fat-soluble components, extract with ethyl acetate with the same volume as the crude extract, extract 4 times in total, and concentrate the ethyl acetate extract under reduced pressure to obtain Extraction, after dissolving the extract with 20% volume concentration ethanol, use D101 macroporous adsorption resin to pack the column for separation, and then use 6 times the column volume of water, volume concentration 10%, 20%, 30%, 40%, 50% %, 60%, 70%, 80%, 90%, and 100% ethanol were eluted, and the eluted parts with a v...

Embodiment 3

[0043]Uncaria stems are dried and crushed, weighed 100g, soaked in 1000mL volume concentration 70% ethanol for 12h, ultrasonicated for 30min, filtered, and the filtrate was stored for later use; repeat the above extraction process 5-6 times, combined the filtrate, concentrated under reduced pressure to remove ethanol to obtain the crude extract of Uncaria. Extract with the same volume of petroleum ether as the crude extract, extract 4 times in total, remove the fat-soluble components, extract with ethyl acetate with the same volume as the crude extract, extract 4 times in total, and concentrate the ethyl acetate extract under reduced pressure to obtain Extract, after dissolving the extract with 20% ethanol by volume concentration, pack the column with AB8 macroporous adsorption resin for separation, and then use 6 times column volume of water, volume concentration 10%, 20%, 30%, 40%, 50% %, 60%, 70%, 80%, 90%, and 100% ethanol were eluted, and the eluted parts with a volume co...

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Abstract

The invention discloses a method for enriching and purifying four alkaloid components with similar structural properties in uncaria rhynchophylla, and belongs to the field of development and utilization of effective components. The method for enriching and separating the compounds in the uncaria comprises the following main process steps: crushing a dried uncaria medicinal material, carrying out ultrasonic extraction with 60-80% ethanol in an amount which is 5-10 times that of the uncaria medicinal material, degreasing with petroleum ether, extracting with ethyl acetate, and carrying out vacuum concentration on an extract liquid to obtain an extract; and preliminarily purifying the obtained extract through macroporous adsorption resin, and further purifying through reversed-phase semi-preparative high performance liquid chromatography to obtain four alkaloid components with the content of 90% or above. The alkaloid compounds in the uncaria are enriched and purified by adopting multiple column chromatography technologies, the process is simple and easy to operate, the content of alkaloids in the finished product is high, all the used resin can be recycled, and industrialization is easy.

Description

technical field [0001] The invention relates to alkaloids, specifically a method for enrichment and purification of four alkaloid components with similar structural properties in Uncaria uncaria. Background technique [0002] Uncaria ( Uncariarhynchophylla (Miq.) Miq.exHavil.) The active ingredients are mainly alkaloids, mainly including rhynchophylline, isorhynchophylline, dehydrorhynchophylline, isodehydrorhynchophylline and other ingredients, which have a wide range of pharmacological effects and have the functions of clearing heat and calming Liver, wind and spasm-relieving effects, also have significant curative effect on cardiovascular diseases, central nervous system diseases and other aspects. In recent years, studies have shown that total alkaloids of Rhycarpa have protective effects on neurons, can improve the learning ability of Alzheimer's disease (AD) patients, and have special therapeutic effects on leukemia and AIDS. The content of alkaloid compounds in Unca...

Claims

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Application Information

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IPC IPC(8): C07D471/20
CPCC07D471/20
Inventor 袁晓艳王明阳李姿瑶
Owner ZUNYI MEDICAL UNIVERSITY