A kind of hexapeptide and its application and pharmaceutical composition

A composition and drug technology, applied in the field of biomedicine, can solve the problems of limited clinical application range, active ingredients, drug mechanism of action, etc., and achieve the effect of promoting skin wound healing and inhibiting Escherichia coli

Active Publication Date: 2022-06-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since DECB is a multi-component biochemical drug, its active ingredients and drug mechanism of action are not clear, and its clinical application range is limited. In 2019, the sales volume was 93 million, showing a downward trend

Method used

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  • A kind of hexapeptide and its application and pharmaceutical composition
  • A kind of hexapeptide and its application and pharmaceutical composition
  • A kind of hexapeptide and its application and pharmaceutical composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Effects of hexapeptides on respiratory activity

[0037] The specific method is as follows:

[0038] Using the 2015 edition of "Chinese Pharmacopoeia" respiratory activity determination method:

[0039] 1. Guinea pig feeding and preparation of liver homogenate

[0040] SPF grade male guinea pigs were raised in SPF animal rooms, the ambient temperature was 23°C±3°C, the air humidity was 50%±5%, and the airflow value was 0.13-0.18m 3 / s, the number of air changes is 10 to 20 times / hour. When the guinea pig weighs 250-300g, the respiratory activity assay can be carried out.

[0041] 2. Measurement of respiratory activity After fasting for 24 hours, the guinea pigs were killed by neck blow method, the carotid artery was immediately cut off for bloodletting, and the liver was dissected and taken out. Wash 2 times in Soerensen buffer. 5.5 g of liver was divided into 7 equal sized pieces, immediately placed in Soerensen buffer, and placed on an ice-water bath. The liver ...

Embodiment 2

[0059] Effects of DECB-6P on energy metabolism of HT22 cells

[0060] 1. The effect of DECBI-6P on mitochondrial membrane potential of HT22 cells

[0061] Mitochondria are power factories, many biochemical reactions, and energy-generating sites, and changes in mitochondrial membrane potential are closely related to cellular energy metabolism. JC-1 is an ideal lipophilic fluorescent probe for detecting mitochondrial membrane potential. When the mitochondrial membrane potential is high, JC-1 exists in the form of polymers in mitochondria, producing red fluorescence; when the mitochondrial membrane potential is low, it exists in the form of monomers and produces green fluorescence. Therefore, the level of mitochondrial membrane potential can be reflected by the ratio of mitochondrial red fluorescence and green fluorescence. The HT22 cell line is a mouse hippocampal neuron cell line, which will adhere to the wall under normal conditions. This cell line is a good cell model for s...

Embodiment 3

[0072] Migration effect of DECB-6P on HaCaT cells

[0073] Use a marker pen to draw horizontal lines evenly on the back of the 6-well plate, about every 0.5-1 cm, across the holes. Pass 5 lines through each hole. In a 6-well plate containing complete culture medium (DMEM high-glucose minimal medium, 100kU / L penicillin, 100mg / L streptomycin, 100mL / L heat-inactivated fetal bovine serum), add about 5×10 6 HaCaT cells were confluent overnight. On the second day, use a yellow pipette tip to scratch the horizontal line perpendicular to the back of the ruler, wash the cells 3 times with PBS, remove the scratched cells, add serum-free medium and DECB-6P, and the final concentration is 25, 50 , 100, 200, 400 μg / mL at 37°C, 5% CO 2 Incubator culture, 24h sampling, calculation of migration rate. Figure 4 Compared with the migration rates of hexapeptide HaCaT cells with different concentrations in Table 5, it can be seen from the results that hexapeptide can promote the migration of ...

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Abstract

The invention provides a hexapeptide and its application and pharmaceutical composition, which belong to the technical field of biomedicine. The hexapeptide is composed of any three or more of serine, valine, proline, isoleucine and asparagine amino acid composition. The hexapeptide provided by the invention has a regulating effect on the energy metabolism of HT22 cells, has a migration effect on HaCaT cells, can inhibit Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, and has a promoting effect on skin wound healing.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to a hexapeptide and its application and pharmaceutical composition. Background technique [0002] The energy metabolism of organisms is closely related to mitochondria. Mitochondria are the site of many biochemical reactions that generate energy. Changes in mitochondrial membrane potential are closely related to cellular energy metabolism. AMP-activated protein kinase (AMPK) is a major potent sensor of cellular energy state in all eukaryotes and is highly conserved in all eukaryotic species. AMPK is closely related to blood circulation dysfunction. For example, during ischemia, the expression of adenosine triphosphate synthase delta subunit (ATP5D) in the mitochondria of cells is reduced due to ischemia and hypoxia at the end of the blocked or blocked blood vessels, resulting in reduced adenosine triphosphate (ATP) synthesis. In addition, the blood vessels and their...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K38/08A61P17/02A61P31/04
CPCC07K7/06A61P31/04A61P17/02A61K38/00Y02A50/30
Inventor 张桂荣张词嘉时亚男胡美娜
Owner JILIN UNIV
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