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Method for promoting proliferation of intestinal cancer cells based on CCL20 and CXCL8

A technology for intestinal cancer cells and cell proliferation, which is applied in the direction of gastrointestinal cells, tumor/cancer cells, animal cells, etc., can solve problems such as the influence of intestinal cancer cell proliferation, and achieve the effect of increasing accuracy

Pending Publication Date: 2021-06-01
云南省肿瘤医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the existing defects and provide a method for promoting the proliferation of intestinal cancer cells based on CCL20 and CXCL8, so as to solve the problem of rarely analyzing the influence of chemokines on the proliferation of intestinal cancer cells proposed in the above-mentioned background technology. question

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for promoting the proliferation of intestinal cancer cells based on CCL20 and CXCL8, comprising the following steps:

[0037] Step 1: Select appropriate experimental materials; Step 2: Prepare the main reagents and store the prepared reagents; Step 3: Proliferate the cells, and perform different experimental tests on the proliferated cells; Step 4: Perform trypan blue on the cells Dyeing experiment, and calculate the cell survival rate; Step 5: Immunohistochemical staining of the cells; Step 6: Select suitable cells, conduct MTT test on the cells, and calculate the cell proliferation rate;

[0038] Step 7: Analyze the experimental data results, and use statistics to process the data.

[0039] In the present invention, preferably, in step 1, a suitable human colon adenocarcinoma cell line is selected from the cell bank of Shanghai Institute of Cell Biology, Chinese Academy of Sciences, and the cells are recovered.

[0040] In the present invention, preferably, ...

Embodiment 2

[0049] A method for promoting the proliferation of intestinal cancer cells based on CCL20 and CXCL8, comprising the following steps:

[0050] Step 1: Select appropriate experimental materials; Step 2: Prepare the main reagents and store the prepared reagents; Step 3: Proliferate the cells, and perform different experimental tests on the proliferated cells; Step 4: Perform trypan blue on the cells Dyeing experiment, and calculate the cell survival rate; Step 5: Immunohistochemical staining of the cells; Step 6: Select suitable cells, conduct MTT test on the cells, and calculate the cell proliferation rate;

[0051] Step 7: Analyze the experimental data results, and use statistics to process the data.

[0052] In the present invention, preferably, in step 1, a suitable human colon adenocarcinoma cell line is selected from the cell bank of Shanghai Institute of Cell Biology, Chinese Academy of Sciences, and the cells are recovered.

[0053] In the present invention, preferably, ...

Embodiment 3

[0062] A method for promoting the proliferation of intestinal cancer cells based on CCL20 and CXCL8, comprising the following steps:

[0063] Step 1: Select appropriate experimental materials; Step 2: Prepare the main reagents and store the prepared reagents; Step 3: Proliferate the cells, and perform different experimental tests on the proliferated cells; Step 4: Perform trypan blue on the cells Dyeing experiment, and calculate the cell survival rate; Step 5: Immunohistochemical staining of the cells; Step 6: Select suitable cells, conduct MTT test on the cells, and calculate the cell proliferation rate;

[0064] Step 7: Analyze the experimental data results, and use statistics to process the data.

[0065] In the present invention, preferably, in step 1, a suitable human colon adenocarcinoma cell line is selected from the cell bank of Shanghai Institute of Cell Biology, Chinese Academy of Sciences, and the cells are recovered.

[0066] In the present invention, preferably, in...

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Abstract

The invention discloses a method for promoting proliferation of intestinal cancer cells based on CCL20 and CXCL8. The method comprises the following steps: 1, selecting a proper experimental material; 2, preparing a main reagent and storing the prepared reagent; 3, proliferating the cells, and carrying out different experimental tests on the proliferated cells; 4, carrying out trypan blue discharge dyeing experiment on the cells, and calculating the survival rate of the cells; 5, carrying out immunohistochemical staining on the cells. Compared with the prior art, multiple groups of experiments are adopted for comparison, the CCL20 stock solution and the CXCL8 stock solution with different concentrations can be respectively arranged for proliferation culture of the intestinal cancer cells; on one hand, the influence of the chemotactic factor concentration on the proliferation rate of the intestinal cancer cells under the condition of the same chemotactic factor is determined; on the other hand, the influence of a single chemotactic factor and two chemotactic factors on the proliferation rate of the intestinal cancer cells under the same chemotactic factor concentration condition can be determined, and the influence of the chemotactic factor concentration and the chemotactic factor number on the proliferation rate of the intestinal cancer cells can be known.

Description

technical field [0001] The invention belongs to the technical field of intestinal cancer cell proliferation, and in particular relates to a method for promoting the proliferation of intestinal cancer cells based on CCL20 and CXCL8. Background technique [0002] Colorectal cancer is prone to liver metastases. How to predict and prevent colorectal cancer liver metastasis is a worldwide problem. As one of the important components of the tumor microenvironment, chemokines play an important role in CLM. Existing studies have shown that macrophage inflammatory protein 3α and interleukin-8 are most closely associated with CLM. However, the related molecular mechanisms are not yet fully understood. Epithelial-mesenchymal transition is a temporary and reversible process of transition from epithelial cells to mesenchymal cells. This process is broadly defined as changes in the expression of different markers during transition from epithelial to mesenchymal morphology, as well as f...

Claims

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Application Information

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IPC IPC(8): C12N5/09G01N1/30G01N33/50G01N33/569G01N33/68C12Q1/02
CPCC12N5/0693C12N5/0679G01N1/30G01N33/5094G01N33/56966G01N33/6863G01N33/5008C12N2501/21
Inventor 程先硕肖尤川栾利昆杨之斌李云峰董坚夏翠锋
Owner 云南省肿瘤医院
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