Unlock instant, AI-driven research and patent intelligence for your innovation.

CircRNA marker for risk assessment and diagnosis of coronary heart disease as well as detection reagent and kit of circRNA marker

A technology for detection reagents and risk assessment, which is applied in the directions of DNA/RNA fragments, recombinant DNA technology, microbial determination/inspection, etc., can solve the problems that the correlation research of coronary heart disease has not been reported, and achieve the improvement of prevention and diagnosis level, diagnosis and so on. The effect of high accuracy of results

Inactive Publication Date: 2021-06-04
FUJIAN MEDICAL UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the association study between hsa_circ_0001946 and coronary heart disease has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • CircRNA marker for risk assessment and diagnosis of coronary heart disease as well as detection reagent and kit of circRNA marker
  • CircRNA marker for risk assessment and diagnosis of coronary heart disease as well as detection reagent and kit of circRNA marker
  • CircRNA marker for risk assessment and diagnosis of coronary heart disease as well as detection reagent and kit of circRNA marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of hsa_circ_0001946 detection kit

[0031] 1. circRNA biomarkers

[0032] In this embodiment, a circRNA marker for risk assessment and / or diagnosis of coronary heart disease is firstly provided, the circRNA marker is hsa_circ_0001946, and its cDNA sequence is shown in SEQ ID NO:1.

[0033] 2. Design of specific primers for hsa_circ_0001946

[0034] In this example, the amplification primers of hsa_circ_0001946 and the internal reference gene (GAPDH) were designed, and the specific sequences are shown in Table 1.

[0035] Table 1 hsa_circ_0001946 and internal reference gene primer sequence list

[0036]

[0037] 3. Configuration of hsa_circ_0001946 detection kit

[0038] This embodiment provides a kit for risk assessment and / or diagnosis of coronary heart disease, which can quantitatively detect the expression level of hsa_circ_0001946. The kit includes an RNA extraction system, a reverse transcription reaction system and a PCR reaction syste...

Embodiment 2

[0052] Example 2 Application of hsa_circ_0001946 in risk assessment and / or diagnosis of coronary heart disease

[0053] 1. Clinical sample collection

[0054] From July 2017 to September 2019, 110 cases of peripheral blood from 110 patients with coronary heart disease diagnosed by coronary angiography were collected from the First Affiliated Hospital of Fujian Medical University and Union Hospital Affiliated to Fujian Medical University. 110 cases of non-coronary heart disease control peripheral blood from other departments. In addition, 25 cases of peripheral blood and 25 cases of control peripheral blood were collected from the community (Xiadu Community Health Service Center, Cangshan District, Fuzhou City, Fujian Province) from October 2019 to November 2019. All study subjects signed an informed consent form, which was approved by the review committee of Fujian Medical University.

[0055] 2. Collection and storage of peripheral blood mononuclear cells

[0056] ①Separat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of biology, and particularly relates to a circRNA marker for risk assessment and / or diagnosis of coronary heart disease. The circRNA marker is hsa_circ_0001946, the cDNA sequence of the circRNA marker is as shown in SEQ ID NO:1, the circRNA marker has very high sensitivity and specificity to the coronary heart disease, AUC for distinguishing peripheral blood of a patient with the coronary heart disease and peripheral blood of a normal control can reach 0.838, the sensitivity reaches 0.809, and the specificity reaches 0.736. Therefore, the circRNA marker can be used for disease risk assessment of the coronary heart disease, a theoretical reference basis is provided for diagnosis of the coronary heart disease, and the prevention and diagnosis level of the coronary heart disease is improved. Moreover, when the four variables of the circRNA marker, coronary heart disease family history, hypertension and diabetes are combined for conjoint analysis, coronary heart disease diagnosis can be carried out, and the accuracy of the diagnosis result is high. The invention also relates to a detection reagent and a kit of the circRNA marker. The detection reagent and the kit both comprise specific primers aiming at hsa_circ_0001946, and the specific primers comprise a specific upstream primer as shown in SEQ ID NO:2 and a specific downstream primer as shown in SEQ ID NO:3.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a circRNA marker for risk assessment and diagnosis of coronary heart disease, a detection reagent and a kit thereof. Background technique [0002] Coronary atherosclerotic heart disease, commonly known as coronary heart disease, is the leading cause of death worldwide. In China, the proportion of ideal cardiovascular health among adults is currently low, only 0.2%, and there are 11 million patients with coronary heart disease, which is the second leading cause of early death. Although the burden of coronary heart disease is currently a major public health problem, efforts are focused on the treatment of the disease rather than on its prevention. Therefore, shifting the focus from intervention to prevention of coronary heart disease with equal emphasis will be of great significance. [0003] Circular RNA (circular RNA, circRNA) is a newly discovered type of endogenous no...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/113C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 吴思英李煌元黄淑娜孙义曾昭楠林少炜谢骁旭徐兴燕
Owner FUJIAN MEDICAL UNIV