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A Simplified Method for Virus-free and Breeding of Apple Field Seedlings

An apple and field technology, applied in the field of agricultural cultivation, can solve the problems of long cycle, cumbersome breeding technology process, heavy workload, etc., and achieve the effects of improving accuracy, shortening detection cycle and easy acquisition.

Active Publication Date: 2022-02-22
FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technical process of detoxification and breeding of apple field seedlings is relatively cumbersome, and has defects such as long cycle and heavy workload

Method used

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  • A Simplified Method for Virus-free and Breeding of Apple Field Seedlings
  • A Simplified Method for Virus-free and Breeding of Apple Field Seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] A simplified apple field seedling detoxification and breeding method comprises the following steps:

[0064] 1) Pretreatment of plant material: collect 1.8cm long shoots, carry out the first subculture and multiplication, and obtain apple test-tube seedlings;

[0065] 2) Virus-free treatment: select well-growing apple test-tube plantlets, cut out the stem tip I and insert it into the medium containing ribavirin, pre-cultivate it for 10 days at room temperature at 25°C, and after the callus appears, further cultivate and cut out the stem tip II;

[0066] 3) Stem tip regeneration: after the preliminary cultivation of the cut shoot tip II, the stem tip is regenerated until the plant grows to 0.6 cm, the outermost callus is removed, and the thickness of the removed part is 1.0 mm, and then transferred to the culture medium. Second subculture to complete regenerated plants;

[0067] 4) Virus detection of complete regenerated plants: cut the upper part of 1.0 cm of complete ...

Embodiment 2

[0085] A simplified apple field seedling detoxification and breeding method comprises the following steps:

[0086] 1) Pretreatment of plant material: collect 2 cm long shoots, carry out the first subculture and multiplication, and obtain apple test-tube seedlings;

[0087] 2) Virus-free treatment: select well-growing apple test-tube plantlets, cut out the stem tip I and insert it into the medium containing ribavirin, pre-cultivate it at room temperature at 25°C for 12 days, and after the callus appears, further cultivate and cut out the stem tip II;

[0088] 3) Stem tip regeneration: after the preliminary cultivation of the cut shoot tip II, the stem tip is regenerated and the plant grows to 0.7 cm, the outermost callus is removed, and the thickness of the removed part is 1.5 mm, and then transferred to the culture medium. Second subculture to complete regenerated plants;

[0089] 4) Virus detection of complete regenerated plants: Cut out the upper 1.2 cm of complete regener...

Embodiment 3

[0107] A simplified apple field seedling detoxification and breeding method comprises the following steps:

[0108] 1) Pretreatment of plant material: collect 2.2cm long shoots, carry out the first subculture and multiplication, and obtain apple test-tube seedlings;

[0109] 2) Virus-free treatment: select well-growing apple test-tube plantlets, cut out the stem tip I and insert it into the medium containing ribavirin, pre-cultivate it at room temperature at 25°C for 13 days, and after the callus appears, further cultivate and cut out the stem tip II;

[0110] 3) Stem tip regeneration: after the preliminary cultivation of the cut shoot tip II, the stem tip is regenerated until the plant grows to 0.8 cm, the outermost callus is removed, and the thickness of the removed part is 2.0 mm, and then transferred to the culture medium. Second subculture to complete regenerated plants;

[0111]4) Virus detection of complete regenerated plants: cut the upper 1.5 cm of complete regenerat...

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Abstract

The invention discloses a simplified virus-free and breeding method for apple field seedlings. It belongs to the technical field of agricultural cultivation. The method comprises the following steps: pretreatment of plant materials, detoxification treatment, stem tip regeneration, virus detection of regenerated plants and breeding of non-toxic apple seedlings. The invention utilizes tender apples for tissue culture, and the material is easy to obtain, which shortens the formation time of test-tube seedlings; improves the heat-resistant ability of the plants, reduces the death rate; effectively shortens the detection period of the plants; omits the link of propagation; effectively The accuracy of detection is greatly improved; the time for plant pretreatment is shortened, and the materials are fully utilized; the time for rooting and transplanting and corresponding cost consumption are saved; different needs can be fully met.

Description

technical field [0001] The invention relates to the technical field of agricultural cultivation, in particular to a simplified method for detoxification and breeding of apple field seedlings. Background technique [0002] Apples are mainly reproduced through asexual methods such as grafting, which has caused widespread infection of apples planted in my country. Once the virus is infected, it will be carried for life, resulting in low yield and poor quality of apples. [0003] At present, the cultivation area and output of apples in my country rank among the top in the world, but the proportion of the non-toxic area is still very small, making it difficult to obtain high profits and occupy the international market. [0004] Therefore, non-toxic cultivation of apples is the general trend. The existing technical process of detoxification and breeding of apple field seedlings is cumbersome, and has defects such as long cycle and heavy workload. [0005] Therefore, it is an urg...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 胡国君董雅凤张尊平范旭东任芳
Owner FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI