Facial cleanser containing a time rhythm regulating composition and a preparation method of facial cleanser
A technology for adjusting time and facial cleanser, which is applied in the direction of medical preparations containing active ingredients, drug combinations, skin care preparations, etc. It can solve problems such as unclear formula mechanism and failure to fundamentally solve skin problems, and restore skin luster , Improve cell metabolism and increase skin moisture
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preparation example 1
[0074] This preparation example provides a hydrolyzed yeast protein, which is prepared by the following preparation method:
[0075] (1) inoculate the brewer's yeast strain into the activated medium, and carry out fermentation and cultivation under the conditions of 28° C. and pH 5 for 40 hours to obtain yeast milk;
[0076] The activation medium comprises: molasses 10g / L, yeast extract 12g / L, peptone 5g / L, ammonium sulfate 4g / L and potassium dihydrogen phosphate 4g / L in terms of mass concentration;
[0077] (2) Concentrate the yeast milk obtained in step (1) until the concentration of the yeast milk is 12wt%, ultrasonically treat at 35°C with a power of 500W for 25min, heat up to 100°C and boil for 20min to obtain the yeast self-solution;
[0078] (3) The yeast self-solution obtained in step (2) is mixed with the composite protease, the quality of the composite protease is 0.1% of the yeast self-solution quality, after the enzymolysis reaction of 18h at 55°C, after inactivati...
preparation example 2
[0081] This preparation example provides a kind of rosemary leaf extract, and described rosemary leaf extract is prepared by following preparation method:
[0082] (a) sieve after rosemary leaf pulverization, obtain 60 order rosemary leaf powder;
[0083] (b) Mix rosemary leaf powder with glass beads of equal volume and place in an extraction kettle. Set the temperature of the extraction kettle to 45°C and the pressure to 5.5MPa; wherein, carbon dioxide is pressurized by a plunger pump (flow rate is 0.6L / min), and ethanol is pumped in by a double-piston reciprocating pump (flow rate is 3L / min). The rosemary leaf powder is extracted, and the extraction time is 80 minutes. The extract is collected from the bottom of the separation kettle and concentrated to obtain the rosemary leaf extract.
preparation example 3
[0085] This preparation example provides a kind of chrysin, which is prepared by the following preparation method:
[0086] (A) Mix 10 parts of propolis and 45 parts of silica gel immobilized ionic liquid, grind evenly, and put it into a glass column after obtaining the mixture; wherein, the preparation method of the silica gel immobilized ionic liquid is: put silica gel Dry it in an oven at 100°C for 3h, then immerse it in 200mM methanol solution of 1-dodecyl-3-methylimidazole, stir the mixture at room temperature for 12h, then dry it in an oven at about 150°C To constant weight, the resulting white powder is the silica gel-supported ionic liquid;
[0087] (B) After using 1200 parts of n-hexane to rinse, then use 1200 parts of methanol to elute, and the eluate is concentrated and then purified on a polyamide reverse-phase column to obtain pure chrysin (the HPLC detection purity is 95.6 %).
[0088] Except hydrolyzed yeast protein, rosemary leaf extract and chrysin were prep...
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