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Vaccine for protection against streptococcus suis

A technology of Streptococcus suis and vaccines, which is applied in the direction of veterinary vaccines, vaccines, antibacterial drugs, etc., and can solve the problems of short-term, failure to meet registration standards, and low level of heterologous protection

Pending Publication Date: 2021-06-15
INTERVET INT BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this passive protection is only very short-lived
On the heels of this, said vaccine has never been registered for protection of offspring by ingestion of colostrum against any other serotype of S. suis bacteria, so apparently the level of heterologous protection is too low to meet the registration criteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The aim of the first study was to test the IgM protease antigen, in this case that of S. suis serotype 2, against conventional bacterin vaccines containing inactivated serotype 2 S. suis bacteria (see PorcilisStrepsuis ) compared to whether it can provide protection against serotype 2 Streptococcus suis challenge.

[0029] Research design

[0030]Thirty weaned pigs were used. Pigs were allocated into three groups of 10 pigs each (evenly distributed across different litter ranges). Group 1 was treated with 230 μg per dose of recombinant rIdeSsuis IgM protease antigen (Seele et al: Vaccine33 as determined by Bradford protein assay using BSA as standard) in oil-in-water adjuvant at 5 and 7 weeks of age. :2207-2212; May 5, 2015, Section 2.2.) Two intramuscular vaccinations. Group 2 was vaccinated intramuscularly twice at 5 and 7 weeks of age with whole-cell bacterin of serotype 2 (see Porcilis Strepsuis) in oil-in-water adjuvant (positive control). Group 3 was not vacci...

Embodiment 2

[0038] The aim of the second study was to test whether the IgM protease antigen could confer protection against challenge with serotype 14 S. suis.

[0039] Research design

[0040] The design of the study was largely the same as that of the first study. Groups of 10 pigs were used and vaccinated twice at 5 and 7 weeks of age with IgM protease antigen (group 1 ) or as unvaccinated control animals (group 2). At 9 weeks of age, pigs were challenged with a highly pathogenic culture of S. suis serotype 14.

[0041] result

[0042] Also in this study, the vaccine did not induce any unacceptable local or systemic reactions. On the day of initial vaccination (5 weeks of age), most pigs had (maternal) antibody titers of approximately 4 log 2 . After vaccination, the vaccine group showed a good antibody response after booster immunization, with an average antibody titer of 9.3log2. Antibody titers in control animals remained low, with an average antibody titer of 4.1 log2. Post-c...

Embodiment 3

[0048] Since protection against S. suis in pigs is preferably obtained during the risk period (typically 4-7 weeks of age), a vaccine containing IgM protease was evaluated as a single dose at 3 weeks of age in maternally derived anti-S. suis positive pigs. Whether the vaccine is effective.

[0049] Research design

[0050] The study design was similar to that of the previous two studies, with the main difference being that 3-week-old anti-Ssuis MDA-positive piglets were vaccinated instead of 5-week-old animals (only 1 in 10 animals appeared to have less than MDA level of detection limit). Group 1 was inoculated once intramuscularly with IgM protease antigen in oil-in-water adjuvant. Group 2 served as the negative challenge control group. At 4 weeks of age, piglets were weaned. At 6 weeks of age, piglets were transported to the challenge chamber and challenged immediately. There was no acclimatization period between shipping and challenge to simulate natural stress. Pigle...

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Abstract

The present invention pertains to a vaccine comprising an IgM protease antigen of Streptococcus suis, for use in a method for protecting pigs against an infection with Streptococcus suis of serotype 2 and against an infection with Streptococcus suis of serotype 14.

Description

technical field [0001] The present invention relates to the protection of pigs against pathogenic infections of various serotypes of Streptococcus suis bacteria. Background technique [0002] Streptococcus suis (S. suis) is one of the main pathogens of contagious bacterial disease in pigs. The pathogen can cause a variety of clinical syndromes, including meningitis, arthritis, pericarditis, polyserositis, sepsis, pneumonia, and sudden death. S. suis is a Gram-positive facultative anaerobic coccus originally defined as Lancefield group R, S, R / S, or T. Later, a new typing system based on type-specific capsular polysaccharide antigens located in the cell wall was proposed. This resulted in a system comprising 35 serotypes (Rasmussen and Andresen, 1998, "16S rDNA sequence variations of some Streptococcus suis serotypes", Int. J. Syst. Bacteriol. 48, 1063-1065), of which serotypes 2, 9 , 1, 7 and 1 / 2 are the most prevalent, while serotype 14 is particularly found in Europe. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/09A61P31/04A61K39/00
CPCA61K39/092A61K2039/552A61P31/04
Inventor A·A·C·雅各布斯
Owner INTERVET INT BV