Fermentation auxiliary material special for ruminants, feed and preparation method
A technology for ruminants and auxiliary materials, applied in the field of feed science and microbial fermentation, can solve the problem of microbial fermentation feed with poor feeding effect, and achieve the effect of improving fermentation flavor and feed nutritional value, nutritional indicators and high nutritional value.
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[0029] In the present invention, the raw material of the fermentation auxiliary material includes 10-15 parts of probiotic starter, and the specific weight part of probiotic starter is 10, 11, 12, 13, 14 or 15 parts. In the present invention, the number ratio of live bacteria of Pichia pastoris, Bacillus subtilis, Bacillus coagulans, Enterococcus faecalis and Lactobacillus plantarum in the probiotic starter is preferably (25-35): (5-15): (5~15): (15~25): (15~25); more preferably (28~32): (8~12): (8~12): (18~22): (18~22) , more preferably 30:10:10:20:20. The present invention has no special limitation on the sources and preparation methods of the Pichia pastoris, Bacillus subtilis, Bacillus coagulans, Enterococcus faecalis and Lactobacillus plantarum, which can be obtained by using conventional commercial products in the field or by self-preparation. In the present invention, the probiotic starter is preferably a liquid preparation, and the probiotic starter preferably uses wa...
Embodiment 1
[0043] Taking a dairy farm in Hebei as an example, the raw material composition of fermentation auxiliary materials is shown in Table 1.
[0044] Among them fermented probiotics:
[0045] Including probiotics: Pichia pastoris, Bacillus subtilis, Bacillus coagulans, Enterococcus faecalis and Lactobacillus plantarum, the ratio of live bacteria is 30:10:10:20:20, and the total number of live bacteria is 100 billion / kg. Among them, Bacillus subtilis (GDM1.372) was obtained from Guangdong Microbial Culture Collection Center; Bacillus coagulans, Enterococcus faecalis and Pichia pastoris were purchased from Guangxi Ubit Biotechnology Co., Ltd.
[0046] Nutrient matrix: 2wt.% glucose, 1wt.% peptone, 0.5wt.% yeast powder, 0.5wt.% sodium chloride and 0.1wt.% dipotassium hydrogen phosphate.
[0047] The obtained fermented probiotics are prepared by mixing the above bacterial species, nutrient matrix and water.
[0048] Brewer's grains: from Yanjing Brewery
[0049] Table 1 Raw materia...
Embodiment 2
[0056] Taking a dairy farm in Guangdong as an example, the raw material composition of fermentation auxiliary materials is shown in Table 2.
[0057] Among them fermented probiotics:
[0058] Including probiotics: Pichia pastoris, Bacillus subtilis, Bacillus coagulans, Enterococcus faecalis and Lactobacillus plantarum, the ratio of live bacteria is 30:10:10:20:20, and the total number of live bacteria is 100 billion / kg. Among them, Bacillus subtilis (GDM1.372) was obtained from Guangdong Microbial Culture Collection Center; Bacillus coagulans, Enterococcus faecalis and Pichia pastoris were purchased from Guangxi Ubit Biotechnology Co., Ltd.
[0059] Nutrient matrix: 2% glucose, 1% peptone, 0.5% yeast powder, 0.5% sodium chloride and 0.1% dipotassium hydrogen phosphate.
[0060] The obtained fermented probiotics are prepared by mixing the above bacterial species, nutrient matrix and water.
[0061] Brewer's grains: from Yanjing Brewery
[0062] Table 2 Raw material compositi...
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