Establishment method of novel hepatic fibroblast strain

A technology for establishing liver fibroblasts and establishing methods, which is applied in the field of establishing new liver fibroblast cell lines, can solve the problems of unsuitable research on the mechanism of occurrence and development of liver metastases from colon cancer, and the detection method of cell purity is not objective enough, and achieves strong intuition. , the effect of strong objectivity

Pending Publication Date: 2021-06-18
刘艳坤
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Problems solved by technology

[0007] However, the existing method for establishing fibroblasts derived from liver fibrosis tissue has the disadvantage that the detection method of cell purity is not objective enough. Th

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  • Establishment method of novel hepatic fibroblast strain
  • Establishment method of novel hepatic fibroblast strain
  • Establishment method of novel hepatic fibroblast strain

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Embodiment

[0052] Such as figure 1 Shown, the establishment method of a kind of novel hepatic fibroblast cell line, comprises the following steps:

[0053] S1, sterile medium preparation: prepare two tubes of 20ml DMEM / F12 sterile medium, which contains 10% fetal bovine serum and 1% penicillin streptomycin, that is, add 10% of DMEM / F12 sterile medium % fetal bovine serum, 100u / ml penicillin sodium and 100u / ml streptomycin sulfate, sealed and precooled on ice for later use;

[0054] S2. Separation of tissues: select liver metastases from patients with liver metastases from colon cancer and normal liver tissues > 5.0 cm from the tumor edge, put them into two tubes of DMEM / F12 medium prepared in advance, and leave the tissues in vitro for 20 minutes brought back to the laboratory by laboratory personnel;

[0055] S3. Tissue cleaning: In the ultra-clean bench, wash the three-dimensional tissue with 1xPBS containing 1% penicillin-streptomycin solution for 3 times to remove necrotic tissue a...

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Abstract

The invention relates to the technical field of colon cancer liver metastasis generation mechanism research, in particular to an establishment method of a novel liver fibroblast strain. Liver tumor related fibroblasts and normal liver fibroblasts from the same colon cancer liver metastasis patient are provided and used for researching the occurrence and development mechanism of liver metastasis of colon cancer, can pair each other, and can eliminate tissue heterogeneity caused by individual difference. The establishment method comprises the following steps that S1, a sterile culture medium is prepared; S2, tissues are separated; S3, the tissues are cleaned; S4, the tissues are treated; S5, the tissues are cultured; S6, a cell strain is identified; and S7, the cell strain is named. The establishment method of the novel liver fibroblast strain is high in objectivity, high in intuition, rapid and accurate, and a novel cell model can be provided for fundamental research on occurrence and progress of liver metastasis of the patient with the colon cancer.

Description

technical field [0001] The invention relates to the technical field of research on the mechanism of liver metastasis of colon cancer, in particular to a method for establishing a novel hepatic fibroblast cell line. Background technique [0002] Genetic and cell biology studies have shown that tumor growth is determined not only by tumor cells but also by tumor stroma. Tumor-associated fibroblasts are the most important cell type in a variety of tumor stroma components, and are also important factors for promoting tumor growth and progression [1.Mueller MM,Fusenig NE.Friends or foes-bipolar effects of the tumor stroma in cancer.Nature reviews Cancer, 2004, 4, (11): 839-849.; 2. Tlsty TD, Hein PW. Know thy neighbor: stromal cells can contribute oncogenic signals. Current opinion in genetics & development, 2001, 11, (1): 54-59 .]. For example, the establishment of xenograft tumors derived from human breast cancer cells in mice relies on the presence of human tumor-derived fib...

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Application Information

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IPC IPC(8): C12N5/071G01N33/569G01N21/64G01N15/14
CPCC12N5/0656G01N33/56966G01N21/6428G01N21/6486G01N15/14C12N2509/10C12N2509/00C12N2501/11G01N2021/6432
Inventor 李景武刘艳坤李玉凤郑璇马龙斌
Owner 刘艳坤
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