Metal organic framework material immobilized glucose oxidase and preparation method and application thereof

A technology of glucose oxidase and metal organic framework, applied in immobilized enzymes, biochemical equipment and methods, oxidoreductases, etc., can solve the problem of long time of enzyme @MOF, improve the rate of degrading glucose, optimize the immobilization conditions, the effect of improving temperature stability

Pending Publication Date: 2021-06-18
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, enzyme @MOF is still insufficient in substrate affinity and catalytic efficiency, and it takes a long time to induce enzyme @MOF in aqueous solution

Method used

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  • Metal organic framework material immobilized glucose oxidase and preparation method and application thereof
  • Metal organic framework material immobilized glucose oxidase and preparation method and application thereof
  • Metal organic framework material immobilized glucose oxidase and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Preparation of metal organic framework material immobilized enzyme 3-MPBA / GOx@ZIF-8

[0057] Preparation of 3-MPBA / GOx@ZIF-8: Dissolve 3-MPBA in 50 μL of ethanol, add 1 mL of GOx and PVP with a molecular weight of 8000, mix well, then add 2 mL of 2-MIM with a concentration of 160 mM and 2 mL of zinc nitrate solution with a concentration of 40 mM was mixed evenly so that the final concentration of glucose oxidase in the mixed solution was 0.2 mg / mL, the final concentration of PVP in the mixed solution was 0.1 mg / mL, and 3-mercaptophenylboronic acid was The final concentration in the mixed solution was 0.1 mg / mL, and the mixed solution was left to stand at room temperature for 10 minutes, and then the mixed solution was centrifuged at 8000 rpm for 5 minutes, washed, and dried in vacuum to obtain 3-MPBA / GOx@ZIF- 8.

[0058] Preparation of 3-MPBA@ZIF-8: Dissolve 3-MPBA in 50 μL of ethanol, add 1 mL of deionized water, PVP with a molecular weight of 8000, mix wel...

Embodiment 2

[0062] Example 2: Preparation of metal organic framework material immobilized enzyme 3-MPBA / GOx@ZIF-8

[0063] Dissolve 3-MPBA in 50 μL of ethanol, add 1 mL of GOx and PVP with a molecular weight of 8000, mix well, then add 2 mL of 160 mM 2-MIM and 2 mL of 40 mM zinc nitrate solution and mix well , so that the final concentration of GOx in the mixed solution is 1mg / mL, the final concentration of PVP in the mixed solution is 0.8mg / mL, and the final concentration of 3-mercaptophenylboronic acid in the mixed solution is 0.6mg / mL, the mixed solution After standing at room temperature for 30 minutes, the mixed solution was centrifuged at 8000 rpm for 5 minutes, washed, and dried in vacuum to obtain 3-MPBA / GOx@ZIF-8.

[0064] Figure 4 a and 4b are the TEM images of 3-MPBA@ZIF-8 and 3-MPBA / GOx@ZIF-8, respectively. It can be seen from the comparison that both 3-MPBA@ZIF-8 and 3-MPBA / GOx@ZIF-8 are granular, with a size of about 200 nm, and the particle size of MOFs decreases after ...

Embodiment 3

[0066] Example 3: Preparation of metal organic framework material immobilized enzyme 3-MPBA / GOx@ZIF-8

[0067] Dissolve 3-MPBA in 50 μL of ethanol, add 1 mL of GOx and PVP with a molecular weight of 8000, mix well, then add 2 mL of 160 mM 2-MIM and 2 mL of 40 mM zinc nitrate solution and mix well , so that the final concentration of GOx in the mixed solution is 1.8mg / mL, the final concentration of PVP in the mixed solution is 1.2mg / mL, and the final concentration of 3-mercaptophenylboronic acid in the mixed solution is 1mg / mL, the mixed solution After standing at room temperature for 24 hours, the mixed solution was centrifuged at 8000 rpm for 5 minutes, washed and dried in vacuum to obtain 3-MPBA / GOx@ZIF-8.

[0068] Image 6 a, 6b and 6c are the thermogravimetric analysis images of ZIF-8, 3-MPBA@ZIF-8 and its immobilized enzyme 3-MPBA@ZIF-8, respectively. like Image 6As shown, from 25°C to 211°C, ZIF-8 loses about 6% of its mass due to the evaporation of water molecules i...

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Abstract

The invention provides metal organic framework material immobilized glucose oxidase and a preparation method and application thereof, and belongs to the technical field of material preparation. According to the invention, the glucose oxidase is introduced into a pore structure of a metal organic framework material ZIF-8 formed by metal zinc ions and 2-methylimidazole by adopting a one-step immobilization method, so that one-step rapid immobilization on enzyme is implemented; and the obtained immobilized enzyme 3-MPBA / GOx@ZIF-8 overcomes the defects of free glucose oxidase in the aspects of pH stability, thermal stability, storage stability, urea tolerance and the like, and has good application in visual detection of glucose.

Description

technical field [0001] The invention belongs to the technical field of material preparation, and in particular relates to a metal-organic framework material-immobilized glucose oxidase and a preparation method and application thereof. Background technique [0002] Enzyme is a biological macromolecular catalyst, which has been widely used in medicine, food, chemical industry, agriculture and other fields due to its substrate specificity, selectivity and green chemistry. However, natural enzymes suffer from low thermal and operational stability, narrow optimal pH range, low tolerance to most organic solvents, difficult recovery, and lack of reusability under operational conditions. Furthermore, enzymes generally perform best in homogeneous systems, leading to complex separation steps required to remove enzymes from product mixtures. [0003] Metal-organic frameworks (MOFs) are three-dimensional network structures with high crystallinity and porosity formed by the coordination...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/089C12N9/04G01N33/66
CPCC12N9/0006C12Y101/03004C12N11/089G01N33/66
Inventor 赵蔓王赟韩娟吴嘉聪李媛媛王蕾
Owner JIANGSU UNIV
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