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Method for detecting HPLC specific chromatogram of folium turpiniae

A detection method and technology of Radix Radix are applied in measurement devices, instruments, scientific instruments, etc., which can solve the problems of inability to identify Radix Radix et Rhizoma, and achieve the effect of sufficient theoretical basis.

Pending Publication Date: 2021-06-18
JIANGZI QINGFENG PHARMACEUTICALS INC
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Problems solved by technology

[0008] The above method can only detect the round leaves of Shanxiang in some areas of Jiangxi Province, and cannot be applied to the identification of the round leaves of Shanxiang in other places

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  • Method for detecting HPLC specific chromatogram of folium turpiniae
  • Method for detecting HPLC specific chromatogram of folium turpiniae
  • Method for detecting HPLC specific chromatogram of folium turpiniae

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Embodiment Construction

[0034] The raw materials or reagents used in the following examples can be purchased directly from the market unless otherwise specified. The present invention is described in conjunction with the following embodiments:

[0035] The specific steps of sample preparation and detection operation are as follows:

[0036] 1. Preparation of blank solvent: Take an appropriate amount of 50% methanol to obtain the solution.

[0037] 2. Preparation of the test solution: accurately weigh 0.3g of the medicinal material powder of Cymbidium sinensis, put in a 50ml volumetric flask, accurately add an appropriate amount of 50% methanol solution, ultrasonicate for 60 minutes (250W), cool, and dilute with 50% methanol solution to Scale, pass through a 0.22μm filter membrane, and take the subsequent filtrate.

[0038] 3. Chromatographic conditions: chromatographic column ACE Excel 3C18-PFP column (150mm×4.6mm, 3μm), with acetonitrile (A)-0.5% formic acid aqueous solution (B) as the mobile phas...

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Abstract

The invention relates to a method for detecting an HPLC specific chromatogram of folium turpiniae. According to the method, an ACE Excel 3 C18-PFP high performance liquid chromatographic column is adopted, acetonitrile is used as a mobile phase A, a 0.2-1.0% (V / V) formic acid aqueous solution is used as a mobile phase B, and gradient elution is adopted. According to the method, the method capable of detecting the characteristic chromatograms of turpinia arguta leaves from different producing areas is established, the theoretical basis of the method is sufficient, and the turpinia arguta leaves from different producing areas can be accurately and intuitively identified and distinguished after verification of the method.

Description

technical field [0001] The invention relates to a detection method for the HPLC characteristic spectrum of the round leaves of Shanxiang, which belongs to the technical field of medicine analysis. Background technique [0002] Shanxiangyuan is the root or leaf of Turpinia arguta (Lindl.) Seem. The sharp-pointed mountain fragrant round plant is distributed in Jiangxi, Fujian, Hunan, Guangdong, Hainan, Guangxi, Sichuan, Guizhou and other places. There are large differences in the contents of privetin and rhodolin in various medicinal materials, some areas are lower than the pharmacopoeia standard, and ursolic acid and ellagic acid are also one of its active components, so only two components are used to calibrate their quality It is not comprehensive enough, and the fingerprint method reported in the existing literature cannot be used to study the components of the medicinal materials in different places of origin. The literature method is related to the "Study on the High P...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/36G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N30/36
Inventor 余水平邓双炳刘地发黄冰峰徐滢方礼刘明颖王振张功俊周鹏张九月
Owner JIANGZI QINGFENG PHARMACEUTICALS INC
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