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Total bile acid detection kit and method thereof

A kit and reagent technology, which is applied in the field of reagents for detecting bile acids based on a cyclic enzymatic method, can solve the problems of decreased reagent stability and great influence on enzyme activity, and achieve the effect of taking into account both the preservation effect and the system stability.

Pending Publication Date: 2021-06-22
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even a small number of preservatives have better antiseptic effect under this pH condition, but they have a great impact on the enzyme activity, which will cause the stability of the reagent to decrease, so it is not suitable for use in the TBA detection system based on the cyclic enzyme method

Method used

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  • Total bile acid detection kit and method thereof
  • Total bile acid detection kit and method thereof
  • Total bile acid detection kit and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The preparation of embodiment 1 reagent 1 and reagent 2

[0067] Prepare reagent 1 and reagent 2 according to Table 1 and Table 2, respectively.

[0068] Table 1

[0069]

[0070] Table 2

[0071]

Embodiment 2

[0072] Embodiment 2 The preparation of the reagent combination based on Tertigol

[0073] According to Table 1 and Table 2, the reagent combination of the experimental group and the contrast group for detecting TBA were prepared, wherein the type and content of surfactant and the type and content of antibiotic preservatives are shown in Table 3 below.

[0074] table 3

[0075]

Embodiment 3

[0076] Embodiment 3 microbial challenge experiment

[0077] In order to verify the antiseptic effect of the reagent, a microbial challenge experiment was carried out. The specific steps are as follows:

[0078] Take a well-packaged reagent 2 in the experimental group or the control group, measure 20mL respectively under aseptic conditions and place them in different sterile reagent bottles for later use.

[0079] Inoculate Acremonium acremonium on the improved Martin slant medium and cultivate it for 24-48 hours, wash the lawn with 5mL sterilized physiological saline and dilute, dilute, adjust its concentration and measure its absorbance with a spectrophotometer, take the absorbance A560nm=0.09 ~0.11 of the bacterial liquid, that is, 10 8 cfu / mL acremonium mold suspension, set aside.

[0080] Take 20mL of the above-mentioned weighed reagent, add 0.2ml of Acremonium suspension, mix well, store the sample at room temperature, detect the number of colonies in the reagent at 0, ...

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Abstract

The present invention relates to a TBA detection kit, which comprises a first reagent, wherein the first reagent contains Thio-NAD; a second reagent contains NADH, 3 alpha-HSD, a nonionic surfactant and an antibiotic preservative. Through the combination of the nonionic surfactant and the antibiotic surfactant, the preservation time of the TBA cycle enzyme method detection reagent is prolonged, and the stability of the reagent is not easily reduced. In addition, the invention also relates to a method for detecting TBA.

Description

technical field [0001] The invention relates to the field of total bile acid detection, in particular to a reagent for detecting bile acid based on a circulating enzyme method. Background technique [0002] Total bile acids (Total bile acids, TBA) is the general term for the metabolites of cholesterol in the liver and enterohepatic circulation. It is the main end product of cholesterol metabolism in the body and an important component of bile. plays an important role. In the blood of normal people, the level of TBA is very small, but when the metabolism of TBA in the liver cells is abnormal, TBA will flow back into the blood, and the phenomenon of elevated TBA in serum will occur. Therefore, the serum TBA level can be combined with a number of liver enzyme indicators to assist in the judgment of the occurrence, progression and prognosis of chronic liver disease, cirrhosis, alcoholic liver disease and cholestasis. [0003] Currently, TBA detection kits in clinical use inclu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/32C12Q1/26
CPCC12Q1/26C12Q1/32
Inventor 程文理
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD