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A kit and method for simultaneously extracting organic acids and amino acids

A technology of organic acids and amino acids, applied in the field of chemical analysis, can solve the problems of unprepared diagnosis of urinary calculi and the inability to provide organic acids and amino acids at the same time, and achieve the effect of improving detection efficiency

Active Publication Date: 2022-07-05
杭州度安医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the detection of amino acids and organic acids is of great significance for the diagnosis of urinary stones. However, in the prior art, due to the differences in the structure and properties of amino acids and organic acids, it is often impossible to provide organic acids and amino acids at the same time, resulting in urinary stones. The diagnosis is not ready

Method used

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  • A kit and method for simultaneously extracting organic acids and amino acids
  • A kit and method for simultaneously extracting organic acids and amino acids
  • A kit and method for simultaneously extracting organic acids and amino acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment 1 tert-butyl methyl ether and acidification extraction

[0070] As an extraction reagent, tert-butyl methyl ether has a wide range of applicability and is very suitable for the extraction of polar substances. The targets (citric acid, oxalic acid and cystine) in the sample to be extracted are all highly polar substances, and the chemical structural formula of tert-butyl methyl ether is It is very similar to the target. According to the principle of similar compatibility, tert-butyl methyl ether is considered to be the preferred test; on the other hand, tert-butyl methyl ether is highly volatile, which can greatly save sample concentration time and cost.

[0071] The consideration for acid extraction is that cystine, oxalic acid and citric acid all contain carboxyl groups, which are strong electron withdrawing groups, which are easier to combine with protons in acidic tert-butyl methyl ether, and the target substance and protons in acidic tert-butyl methyl ethe...

Embodiment 2

[0095] Example 2 Other extraction reagents and alkalization extraction

[0096] In order to further screen the extraction reagents that can simultaneously extract the above-mentioned target products, the inventors selected other extraction reagents and tested them according to the above-mentioned failure reasons, and tried the alkalization extraction method at the same time.

[0097] The extraction reagents were ethyl acetate, n-hexane, 0.5% ammonia water·tert-butyl methyl ether, 0.5% ammonia water·ethyl acetate, and 0.5% ammonia water·n-hexane, and tert-butyl methyl ether was used as a control.

[0098] The derivatization steps are the same as those in Example 1.

[0099] After the samples were derivatized, they were dried under nitrogen, and 1 mL of extraction reagents (tert-butyl methyl ether, ethyl acetate, n-hexane, 0.5% ammonia water·tert-butyl methyl ether, 0.5% ammonia water·ethyl acetate, and 0.5% ammonia water·n-hexane) were added, respectively. Sonicate for 15 min,...

Embodiment 3

[0107] Embodiment 3 Optimization of ammonia water content of ammonia water·ethyl acetate extraction reagent

[0108] As can be seen from the results of Example 2, the simultaneous extraction of amino acids and organic acids can be achieved by using basic ethyl acetate as an extraction reagent, but the concentration of ammonia water will affect the extraction efficiency of each target to varying degrees. Therefore, the inventor further optimizes the concentration of ammonia water, In order to achieve the highest comprehensive extraction efficiency. The ammonia content is shown in Table 5:

[0109] Table 5 Optimization of ammonia water and ethyl acetate extraction reagent for ammonia water content

[0110]

[0111] Comprehensive comparison found that 0.2-0.6% ammonia water·ethyl acetate as extraction reagents could effectively extract cystine, oxalic acid and citric acid, and the relative extraction efficiency was higher than 20%. Among them, the relative extraction efficie...

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Abstract

The invention discloses a kit for simultaneously extracting organic acids and amino acids, belonging to the field of chemical analysis. The kit comprises basic ethyl acetate. The present invention further includes a method for simultaneously extracting organic acids and amino acids using the kit. Using the extraction kit and extraction method of the present invention, organic acids and amino acids can be simultaneously extracted from samples, especially urine samples, and the relative extraction efficiency is higher than 20%. The extraction method of the invention only needs ultrasonication, vibration and centrifugation, which is simple, convenient and very fast. When applied to urine detection, the level of organic acids and amino acids in urine can be fully understood, and the detection efficiency can be greatly improved because there is no need for separate detection, which has important clinical application value.

Description

technical field [0001] The invention belongs to the field of chemical analysis, and in particular relates to a kit and method for simultaneously extracting organic acids and amino acids. Background technique [0002] Urine, commonly known as "urine", "urine". are metabolites excreted from the organism. The main component of urine is water, followed by protein, glucose, urea, uric acid, inorganic salts, and a small amount of amino acids and organic acids under normal circumstances. [0003] Due to the high renal threshold for plasma amino acids, there are only small amounts of amino acids in normal urine. However, when there are certain diseases that increase the concentration of amino acids in the blood, it can overflow into the urine, resulting in amino acid urine. Urinary stones can also be caused when the levels of certain amino acids are increased. [0004] Organic acids are intermediate products (carboxylic acids) produced in catabolic processes such as amino acid d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C55/06C07C59/265C07C59/245C07C51/48C07C319/28C07C323/58G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72
CPCC07C51/48C07C319/28G01N30/02G01N30/06G01N30/36G01N30/34G01N30/7233G01N2030/067C07C55/06C07C59/265C07C59/245C07C323/58
Inventor 高飞海云谷云云罗示齐
Owner 杭州度安医学检验实验室有限公司
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