Cryogenic storage equipment without liquid nitrogen replenishment

Abstract

The invention provides cryogenic storage equipment without liquid nitrogen replenishment. The cryogenic storage equipment comprises a sample storage mechanism, a refrigeration mechanism and a walking mechanism. The sample storage mechanism comprises a liquid nitrogen tank, and a sample is placed in the liquid nitrogen tank. The refrigeration mechanism comprises a storage tank and a refrigerator, the storage tank is arranged in the liquid nitrogen tank, and liquid nitrogen is stored in the storage tank. The refrigerator is arranged on the outer side of the liquid nitrogen tank, and the refrigerator liquefies the liquid nitrogen gasified in the storage tank again. The walking mechanism comprises rollers, and the rollers are arranged at the bottom of the liquid nitrogen tank. In this way, the cryogenic storage equipment has the advantages that the biological sample and the liquid nitrogen can be isolated, and meanwhile inconvenience caused by replacement of a replenishment tank can be reduced.

Description

technical field [0001] The invention belongs to the technical field of biological storage containers, and in particular relates to a cryogenic storage device that does not need to be supplied with liquid nitrogen. Background technique [0002] At present, the biological sample storage containers used in the market are mainly divided into gas-phase liquid nitrogen tanks (there is a small amount of liquid nitrogen at -196°C in the tank, and the low-temperature nitrogen below -150°C volatilized by liquid nitrogen is used to store biological samples) and liquid-phase liquid nitrogen. Tank (there is a large amount of liquid nitrogen at -196°C in the tank, and biological samples are stored through the low temperature characteristics of liquid nitrogen). [0003] Since the vitrification temperature of water is -137°C, below this temperature, the damage of water crystallization to biological samples can be ignored, so as to ensure the active preservation of biological samples. Howe...

AI Technical Summary

Problems solved by technology

However, the use of liquid nitrogen to store biological samples has the risk of cross-infection between biological samples after freezing and bursting of cryopreservation tubes. Therefore, gas-phase liquid nitrogen tanks are widely used to store biological samples in the market.

[0004] However, the use of gas-phase liquid nitrogen tanks to store biological samples requires regular replenishment of liquid nitrogen to the gas-phase liquid nitrogen tanks, and the need to regularly check the liquid nitrogen balance in the supply tanks and replace them in time

Moreover, the liquid nitrogen and biological samples in the gas-phase liquid nitrogen tank are not completely isolated, and there is a risk of contamination of biological samples due to excessive filling of liquid nitrogen, especially in case of liquid nitrogen leaking out of the tank, its low temperature characteristics may even damage the biological samples. Irreversible frostbite injury to the operator

In addition, regular human intervention is required to replace the supply tank, and there is a risk that the liquid nitrogen supply will be interrupted due to manual operation errors, which will cause the biological samples to be damaged by high temperature.

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