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A kind of walnut kernel polypeptide and preparation method thereof

A walnut kernel and polypeptide technology is applied in the field of walnut kernel polypeptide and its preparation, which can solve the problems of easy-to-generate microorganisms, produce bitter taste, long time for enzymatic extraction, etc. Effect

Active Publication Date: 2021-10-08
广州市金龟寿药品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the traditional acid and alkali processing, the enzymatic method has incomparable advantages, such as no harmful residues, high specificity and high efficiency, small amount of enzyme preparation, mild reaction conditions, and less loss of nutrients. Easy to operate, low energy consumption, etc., but enzymatic extraction takes a long time, is prone to microorganisms, and will produce bitter taste after enzymatic hydrolysis, so it is urgent to develop a method that can effectively extract and preserve walnut polypeptides

Method used

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  • A kind of walnut kernel polypeptide and preparation method thereof
  • A kind of walnut kernel polypeptide and preparation method thereof
  • A kind of walnut kernel polypeptide and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, walnut kernel polypeptide of the present invention and preparation method thereof

[0025] Recipe: Alkaline protease 10g, papain 10g, Protamex enzyme 10g, walnut kernel polypeptide primary product 10g, chitosan 15g and sodium tripolyphosphate 5g.

[0026] Preparation method: S1. After soaking walnut kernels, peel and homogenize for 10 minutes, adjust the pH value to 7, use ultrasonic-assisted stirring for 1 hour, soak overnight and then centrifuge for 0.5 hours, remove the oil layer and lower sediment, adjust the pH value to 4, and stir statically. Centrifuge for 0.5h after placing, take the precipitated layer, wash it with water until neutral, and freeze-dry it in a vacuum drying oven to obtain walnut protein powder;

[0027] S2. Enzymolyze the walnut protein powder prepared in step S1 under the action of a compound enzyme, the enzymolysis temperature is 45° C., and the enzymolysis time is 30 minutes to obtain an enzymolysis product;

[0028] S3. The enz...

Embodiment 2

[0030] Embodiment 2, walnut kernel polypeptide of the present invention and preparation method thereof

[0031] Formula: Alkaline protease 10g, papain 5g, Protamex enzyme 15g, walnut kernel polypeptide primary product 10g, chitosan 20g and sodium tripolyphosphate 5g.

[0032] Preparation method: S1. After soaking walnut kernels, peel and homogenize for 20 minutes, adjust the pH value to 8, use ultrasonic-assisted stirring for 1.5 hours, soak overnight and then centrifuge for 1 hour, remove the oil layer and lower sedimentation, adjust the pH value to 5, and stir statically. Centrifuge for 0.75h after placing, take the sediment layer, wash it with water until neutral, and freeze-dry it in a vacuum drying oven to obtain walnut protein powder;

[0033] S2. Enzymolyze the walnut protein powder prepared in step S1 under the action of a compound enzyme, the enzymolysis temperature is 50° C., and the enzymolysis time is 45 minutes to obtain an enzymolysis product;

[0034] S3. The e...

Embodiment 3

[0036] Embodiment 3, walnut kernel polypeptide of the present invention and preparation method thereof

[0037] Formula: 20g of alkaline protease, 15g of papain, 25g of Protamex enzyme, 15g of walnut kernel polypeptide primary product, 30g of chitosan and 5g of sodium tripolyphosphate.

[0038] Preparation method: S1. After soaking walnut kernels, peel and homogenize for 30 minutes, adjust the pH value to 8, use ultrasonic-assisted stirring for 2 hours, soak overnight and then centrifuge for 1.5 hours, remove the oil layer and lower sediment, adjust the pH value to 4, and stir statically. Centrifuge for 1 hour after placing, take the sediment layer, wash it with water until neutral, and freeze-dry it in a vacuum drying oven to obtain walnut protein powder;

[0039] S2. Enzymolyze the walnut protein powder prepared in step S1 under the action of a compound enzyme, the enzymolysis temperature is 55° C., and the enzymolysis time is 60 minutes to obtain an enzymolysis product;

...

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Abstract

The invention belongs to the technical field of polypeptide preparation, and specifically discloses a walnut kernel polypeptide and a preparation method thereof. The method for extracting walnut kernel polypeptides by ultrasonic-assisted compound enzymes in the present invention effectively improves the extraction rate of walnut kernel polypeptides, and at the same time prepares the walnut kernel polypeptides into The microparticles can effectively cover the bitterness existing in the enzymatic hydrolysis of walnut kernel polypeptides, inhibit the growth of microorganisms, increase the loading capacity of walnut kernel polypeptides, further enhance the effect of walnut kernel polypeptides, and broaden its application range.

Description

technical field [0001] The invention belongs to the technical field of polypeptide preparation, and relates to a walnut kernel polypeptide and a preparation method thereof. Background technique [0002] my country's walnut resources are very rich, and its output ranks first in the world. Walnuts are of high edible value and have outstanding health care functions. The protein content of walnut kernel is 14%~17%, and it contains 8 kinds of essential amino acids required by the human body, which is close to the standards stipulated by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO), and is a good vegetable protein. Walnut protein contained in walnuts is a very high-quality vegetable protein. In addition to being used in the production of primary food, it can also be used as a food base or food additive, with broad application prospects. At present, walnut polypeptide with high added value has become one of the main proc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K17/10C07K1/26C07K1/14A23L33/18
CPCA23V2002/00A23L33/18C07K1/145C07K1/26C07K17/10C12P21/06A23V2200/30A23V2250/55
Inventor 王中振谢涛谢骞刘家生
Owner 广州市金龟寿药品有限公司