Cell mitochondria separation kit
A technology of mitochondria and kits, applied in biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as inability to achieve rapid separation
Pending Publication Date: 2021-07-06
HENAN POLYTECHNIC UNIV
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Problems solved by technology
[0004] The purpose of the present invention is to solve the problem in the prior art that rapid separation cannot be achieved and the internal temperature of the kit can be effectively controlled during separation, and a cell mitochondrial separation kit is proposed
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[0026] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.
[0027] refer to Figure 1-6 , a cell mitochondrial separation kit, comprising a box body 1, and a centrifugal device and a temperature control device arranged inside the box body 1;
[0028] The centrifugal device includes a servo motor 5, a rotating shaft 6, a centrifugal roller 7, a liquid discharge port 8, a liquid inlet 9 and a stirring assembly; the output shaft of the servo motor 5 is fixedly connected to the bottom of the rotating shaft 6; the outer wall of the rotating shaft 6 is connected to the centrifugal roller 7 The inner wall of the centrifugal roller is fixedly connected; the liquid outlet 8 runs through the side wall of the centrifugal roller 7; the l...
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The invention discloses a cell mitochondria separation kit. The cell mitochondria separation kit comprises a kit body, a centrifugal device and a temperature control device, wherein the centrifugal device and the temperature control device are arranged in the kit body. When the kit is used, a reagent needing to be used is added into a centrifugal roller, at the moment, a servo motor can be controlled to work through a control panel, then a rotating shaft is driven to rotate, the centrifugal roller starts to rotate, and a solution placed in the centrifugal roller is discharged to the inner side wall of a fixed block through a liquid discharging opening through rotation of the centrifugal roller; then the centrifugal roller rotates to drive a fixing rod to rotate, then a stirring shaft is driven to rotate so that the solution discharged through the liquid discharging opening is stirred again through the stirring shaft, then the solution enters the centrifugal roller again through a liquid inlet from the inner side wall of the fixed block which is obliquely arranged, and sufficient centrifugation of the solution is achieved; and the centrifugal efficiency of the reagent is ensured, so that a product obtained by centrifugation can better meet the standard of personnel, and the quality of the product obtained by centrifugation is improved while the time is saved.
Description
technical field [0001] The invention relates to the technical field of mitochondria, in particular to a cell mitochondria separation kit. Background technique [0002] Mitochondria is an organelle surrounded by two membranes that exists in most cells. It is a structure that produces energy in cells and is the main place for cells to carry out aerobic respiration. Its diameter is around 0.5 to 1.0 microns. With the exception of Entamoeba histolytica, Giardia basketlini, and several microsporidia, most eukaryotic cells possess mitochondria to a greater or lesser extent, but the mitochondria they possess differ in size, number, and appearance. All are different. Mitochondria have their own genetic material and genetic system, but their genome size is limited, and they are semi-autonomous organelles. In addition to providing energy for cells, mitochondria are also involved in processes such as cell differentiation, cell information transmission and apoptosis, and have the abi...
Claims
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Patent Timeline
Login to View More IPC IPC(8): C12M1/38C12M1/02C12M1/00
CPCC12M27/02C12M33/10C12M41/24C12M47/04
Inventor 郝双影陈兴颖李金丽袁帅刘志强
Owner HENAN POLYTECHNIC UNIV