A strain of Bacillus subtilis and its fermentation method and application

A technology of Bacillus subtilis and fermentation medium, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as difficult denitrification, achieve simple process, high safety, and effective fast effect

Active Publication Date: 2022-05-17
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nitrification needs to be carried out under aerobic conditions, but there may be low dissolved oxygen in the actual aquaculture process, so it will cause certain difficulties in denitrification. Therefore, the development and application of some new high-efficiency denitrification microbial strains will be Improve the efficiency of traditional biological denitrification process

Method used

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  • A strain of Bacillus subtilis and its fermentation method and application
  • A strain of Bacillus subtilis and its fermentation method and application
  • A strain of Bacillus subtilis and its fermentation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Screening and identification of Bacillus subtillis NX-2C (hereinafter referred to as denitrification strain NX-2C).

[0025] The denitrification strain NX-2C was collected by the inventor in May 2020 at the Jinghu Sludge Institute of Nanjing University of Technology, Nanjing, Jiangsu Province. Its classification name is Bacillus subtillis (Bacillus subtillis), and the strain name is Bacillus subtillis NX-2C. The number is CGMCC NO.21625.

[0026] The screening process is as follows:

[0027] The screening solid medium is: ammonium chloride 5g / L, magnesium sulfate heptahydrate 2g / L, dipotassium hydrogen phosphate 3g / L, sodium chloride 1.5g / L, glucose 20g / L, agar 20g / L.

[0028] Preferably, if a bacterial strain grows on the screening medium, it is judged that the bacterial strain can grow with ammonium chloride as the only nitrogen source.

[0029] To determine whether the denitrification strain NX-2C can grow with sodium nitrite or potassium nitrate as the ...

Embodiment 2

[0039] Example 2 Denitrification effect of denitrification bacterial strain NX-2C in artificially prepared sewage.

[0040] Artificially prepared sewage is: ammonium chloride 0.2g / L, sodium nitrite 0.1g / L, potassium nitrate 0.1g / L, glucose 20 g / L, sodium chloride 1g / L, dipotassium hydrogen phosphate 0.5g / L, Magnesium sulfate heptahydrate 0.1g / L, manganese sulfate 0.2g / L; containing ammonia nitrogen 68mg / L, nitrite nitrogen 67mg / L, nitrate nitrogen 61mg / L.

[0041] Both the denitrification bacterial strain NX-2C and Bacillus subtilis CGMCC NO.1.4436 were 10 4 The final concentration of CFU / mL was added to the artificially prepared sewage as the experimental group, and the control group was added with an equal volume of normal saline, with 3 repetitions in each group.

[0042] Among them, the above-mentioned Bacillus subtilis CGMCC NO.1.4436 was purchased from China General Microorganism Culture Collection and Management Center.

[0043] The ammonia nitrogen detection method a...

Embodiment 3

[0054] Example 3 Denitrification effect of denitrification strain NX-2C at different temperatures.

[0055] The artificial preparation of sewage is the same as in Example 2.

[0056] Both the denitrification bacterial strain NX-2C and Bacillus subtilis CGMCC NO.1.4436 were 10 4 The final concentration of CFU / mL was added to the artificially prepared sewage, and the control group was added with an equal volume of normal saline, with 3 repetitions in each group.

[0057] Under different temperature (5, 10, 15, 20, 25, 30, 35, 40°C) culture conditions, denitrification bacteria strain NX-2C and Bacillus subtilis CGMCC NO.1.4436 were inoculated in 100mL of artificially prepared sewage above at 200rpm / min Shock culture. During this period, the content of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen should be detected every 12 hours, and the detection should be at least 48 hours.

[0058] Table 2 Denitrification rate of denitrification strains NX-2C and NO.1.4436 at dif...

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Abstract

The invention discloses a Bacillus subtilis ( Bacillus subtillis ) NX‑2C, which was deposited in the General Microorganism Center of China Committee for the Collection of Microbial Strains on January 15, 2021, with the preservation number CGMCC NO.21625. The invention also discloses the fermentation method and application of the bacterial strain. The denitrification bacterial strain NX-2C of the present invention has both heterotrophic nitrification ability, simple process, can tolerate high concentration of waste nitrogen in aquaculture water, has quick effect after being put into use, is easy to operate and has no secondary pollution, and can withstand low temperature and low Dissolved oxygen, suitable for short-term denitrification process, and high-efficiency denitrification can also be achieved by using low-concentration denitrification strains.

Description

technical field [0001] The invention relates to the technical field of biological environment microorganisms, and relates to a bacillus subtilis and a fermentation method thereof and the application of denitrification of aquaculture water in aquaculture. Background technique [0002] In the process of aquaculture, the increase of nitrogen content in the aquaculture water will cause different degrees of damage to the aquaculture body, for example, inflammation, hemorrhage, decreased food intake and even death. Therefore, the nitrogen content in aquaculture water is a very important detection index for aquaculture water. The biological denitrification process is nitrification-denitrification, in which nitrification is the transformation of ammonia nitrogen into nitrite nitrogen and then into nitrate nitrogen under the action of nitrifying bacteria; Nitrogen is transformed into nitrogen gas etc. and separated from the water body to achieve a thorough purification process. Nit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C02F101/16C02F101/38
CPCC12N1/20C02F3/34C02F2101/163C02F2101/166C02F2101/38Y02W10/10
Inventor 徐虹程利芳羽雷鹏王瑞孙良李莎
Owner NANJING UNIV OF TECH
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