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Manufacturing method of scale insect specimen

A production method and technology for scale insects, which are applied in the preparation of samples for testing, preservation of human or animal bodies, sampling, etc., can solve the problems of fading, poor quality of specimens, and only suitable for use, so as not to become soft or light. , good observation and preservation, the effect of improving the service life

Pending Publication Date: 2021-07-23
凭祥海关综合技术服务中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing scale insect specimens with better effect, so as to overcome the defects that the specimens produced by the existing specimen preparation methods are of poor quality, will fade seriously after a long storage time, and are only suitable for female insects

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  • Manufacturing method of scale insect specimen
  • Manufacturing method of scale insect specimen
  • Manufacturing method of scale insect specimen

Examples

Experimental program
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Effect test

Embodiment 1

[0033] An improved preparation method of a scale insect specimen, comprising the steps of:

[0034] (1) Killing: kill the scale insects and store them in a mixture of lactic acid and ethanol; the mixture is a mixture of 95% ethanol and 75% lactic acid at a volume ratio of 3:1. Compared with the previous method that only stored in ethanol, the worm body is not easy to become soft and light, which is beneficial to the next operation.

[0035] (2) Dissolution: Open a small slit with ophthalmic tweezers on the right edge of the worm body or laterally on the back or at the right 3 / 4 of the worm body; among them, the winged males first use tweezers to remove the wings and use ophthalmic tweezers to open a small slit. After making a small slit, soak in 10% KOH for 12 to 24 hours to dissolve the contents in the body. The higher the alcohol concentration used in the early stage, the longer the time used here.

[0036] During this period, insect needles can be used to carefully dig out...

Embodiment 2

[0051] An improved preparation method of a scale insect specimen, comprising the steps of:

[0052] (1) Killing: Kill scale insects and store them in a mixture of lactic acid and ethanol; the mixture is a mixture of 95% ethanol and 75% lactic acid at a volume ratio of 2:1. Compared with the previous method that only stored in ethanol, the worm body is not easy to become soft and light, which is beneficial to the next operation.

[0053](2) Dissolution: Open a small slit with ophthalmic tweezers on the right edge of the worm body or laterally on the back or at the right 3 / 4 of the worm body; among them, the winged males first use tweezers to remove the wings and use ophthalmic tweezers to open a small slit. After making a small slit, soak in 10% KOH for 12 to 24 hours to dissolve the contents in the body. The higher the alcohol concentration used in the early stage, the longer the time used here.

[0054] During this period, insect needles can be used to carefully dig out the ...

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Abstract

The invention relates to the technical field of specimen manufacturing methods, and particularly discloses an improved manufacturing method of a scale insect specimen, which comprises the following steps: (1) killing; (2) dissolving; (3) cleaning; (4) dyeing; (5) dehydrating; (6) further dehydrating; (7) transparentizing; (8) fixing; and (9) drying. The scale insect prepared by the method can be preserved for a long time, can be used for better observation and preservation of a specimen, and is suitable for a male individual. Wherein the killed insect is stored in a mixed solution composed of lactic acid and ethanol, and the insect body are not easy to soften or fade, so that the subsequent operation is facilitated. The dyeing agent composed of acidic alcohol and acidic fuchsin is used for dyeing, and the phenomenon of insufficient dyeing or excessive dyeing is avoided. And alcohol and propyl alcohol are adopted for dehydration, so that the service life of the specimen is further prolonged. The method can be used for making male or male and female scale insect specimens.

Description

technical field [0001] The invention belongs to the technical field of specimen preparation methods, in particular to a preparation method of a scale insect specimen. Background technique [0002] Scale insects are one of the groups of insects whose morphological characteristics change more complicatedly. Usually, the insects are small and dioecious. The external features such as the waxy secretions in vitro, the color and shape of the shell are far from enough, it must be made and sorted in the laboratory before its morphological structure can be observed under a microscope. Typically, scale insects are identified by slide specimens taken from adult females. However, scale insects are the collective name of the Scaloidaceae, and the methods used by different families under this superfamily are not the same, and the literature records mostly use female insects to make slides, not necessarily suitable for winged male individuals. At the same time, after the previous method ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N1/34A01N1/00
CPCG01N1/2813G01N1/30G01N1/34A01N1/00
Inventor 刘若思钟勇梁琨马福欢白华菊蓝翊文
Owner 凭祥海关综合技术服务中心